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太婆梨果中挥发性香气成分的SPME-GCMS分析.docx

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太婆梨果中挥发性香气成分的SPME-GCMS分析 Title:AnalysisofVolatileAromaticComponentsinGrannySmithApplesUsingSPME-GCMS Abstract: ThevolatilearomaticcomponentsofGrannySmithappleswereanalyzedusingSolidPhaseMicroextraction-GasChromatographyMassSpectrometry(SPME-GCMS)technique.ThisstudyaimedtoidentifyandquantifythevolatilecompoundsresponsibleforthedistinctfragranceofGrannySmithapples.Theresultsrevealedthepresenceofseveralsignificantcomponents,includingesters,alcohols,aldehydes,andterpenes.Understandingthevolatileprofileofapplescanfacilitatethedevelopmentofqualitycontrolmeasuresandenhancetheflavorandaromaattributesofdifferentapplevarieties. Introduction: Thearomaoffruitsplaysacrucialroleinconsumerpreferenceandacceptance.Amongfruits,apples(Malusdomestica)arewidelyconsumedandappreciatedfortheirdiverseflavorsandcharacteristicaromas.TheGrannySmithapple,apopularcultivar,isgloballyrecognizedforitsdistincttartnessandrefreshingaroma.Thevolatilecompoundsresponsibleforthesesensoryattributeshavegainedsignificantinterestinrecentyears.Therefore,thisstudyaimedtoanalyzethevolatilearomaticcomponentsofGrannySmithapplesusingtheSPME-GCMStechnique. Methods: 1.Samplepreparation:FreshGrannySmithapplesampleswereselectedandthoroughlywashed.Theappleswerepeeled,sliced,andhomogenizedtoensureuniformity. 2.SPME-GCMSextraction:AnSPMEfibercoatedwithpolydimethylsiloxane(PDMS)wasusedfortheextraction.Thefiberwasexposedtotheheadspaceofthesampleforaspecifiedtimetoallowfortheabsorptionofvolatilecomponents.ThefiberwastheninsertedintotheGCinjectorportforthermaldesorption. 3.GCMSanalysis:Agaschromatographcoupledwithamassspectrometer(GCMS)wasemployedtoseparateandidentifytheextractedvolatilecompounds.Thecolumnwastemperature-programmedtomaximizeresolution.Massspectrometryprovidedinformationonthemass-to-chargeratio(m/z)andrelativeabundanceofthecompounds,whichfacilitatedtheiridentification. Results: TheSPME-GCMSanalysisoftheGrannySmithapplearomarevealedabroadarrayofvolatilecompounds.Themajorvolatilecomponentsidentifiedincludedesters