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基于高通量测序的不同窖龄窖泥微生物结构与多样性分析.docx

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基于高通量测序的不同窖龄窖泥微生物结构与多样性分析 Title:AnalysisofMicrobialStructureandDiversityinDifferentAgingCellarsusingHigh-ThroughputSequencing Abstract: Inrecentyears,thestudyofmicrobialcommunitiesinvariousenvironmentshasgainedsignificantattention,includingthemicrobialstructureanddiversityintraditionalagingcellars.Thisstudyaimstoinvestigatethemicrobialstructureanddiversityindifferentagingcellarsusinghigh-throughputsequencing.Sampleswerecollectedfrommultiplecellarswithvaryingagingdurations,andthemicrobialprofileswereanalyzedusingnext-generationsequencingtechniques.Theresultsprovideinsightsintothecompositionanddiversityofmicroorganismsinagingcellarsandtheirpotentialrolesintheagingprocessofvariousproducts. Introduction: Traditionalagingcellarsareknowntoharborcomplexmicrobialcommunitiesthatcontributetothematurationanddevelopmentofdifferentagedproductssuchaswines,cheeses,andspirits.Themicrobialcompositionanddiversityinthesecellarscansignificantlyimpactthesensorycharacteristicsandqualityofthefinalproduct.Withtheadvancementofhigh-throughputsequencingtechnologies,itisnowpossibletoexplorethemicrobialstructureanddiversityinthesecellarsingreatdetail. Methods: 1.Samplecollection:Sampleswerecollectedfromagingcellarswithdifferentagingdurations.Carefulconsiderationwasgiventomaintainasepticconditionsduringthecollectiontominimizecontamination. 2.DNAextraction:MicrobialDNAwasextractedfromthecollectedsamplesusingaDNAextractionkitspecificallydesignedformicrobiomestudies.TheextractionprocesswascarefullyoptimizedtomaximizeDNAyieldwhileminimizingpotentialbiases. 3.Librarypreparation:TheextractedDNAsampleswereprocessedtocreatesequencinglibrariesusingaPCRamplification-basedapproach.Barcodedprimerstargetingthe16SrRNAgene(bacterialcommunity)andITSregion(fungalcommunity)wereusedtogenerateamplicons. 4.High-throughputsequencing:ThepreparedlibrariesweresequencedusingIlluminaMiSeqorothernext-generationsequencingplatforms.Thesequencingdepthwasdeterminedtoensuresufficientcoverageforaccurateanalysis. 5.Bioinformatics