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基于Au纳米团簇荧光猝灭特性检测酪氨酸酶活性（英文）.docx

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基于Au纳米团簇荧光猝灭特性检测酪氨酸酶活性（英文） Title:DetectionofTyrosinaseActivityBasedonAuNanoclustersFluorescenceQuenchingProperties Abstract: Enzymesplayacrucialroleinbiologicalsystems,includingcatalyzingbiochemicalreactions.Theactivityofenzymescanbeevaluatedusingvariousmethods.Inthisstudy,weproposeanewapproachtodetecttyrosinaseactivitybasedonthefluorescencequenchingpropertiesofAunanoclusters(AuNCs). Introduction: Tyrosinaseisacopper-containingenzymethatplaysacriticalroleinmelaninbiosynthesis.Itsactivitycanbeusedasanindicatorforvariousapplications,includingdiseasediagnosticsanddrugdevelopment.Traditionalmethodsforassessingtyrosinaseactivity,suchasUV-Visspectroscopyandhigh-performanceliquidchromatography(HPLC),havelimitations,suchastime-consumingproceduresandexpensiveinstruments. Methods: ThisstudyproposesanovelmethodfordetectingtyrosinaseactivityusingAunanoclusters.Aunanoclusters,duetotheiruniqueopticalproperties,havegainedsignificantattentioninbiosensingapplications.ThesynthesisofAunanoclusterswascarriedoutusingasimpleone-potmethod.Aftersynthesis,theAunanoclusterswerecharacterizedusingvarioustechniques,suchastransmissionelectronmicroscopy(TEM),dynamiclightscattering(DLS),andUV-Visspectroscopy. Results: ThesynthesizedAunanoclustersexhibitedstrongfluorescenceemissionataround660nm.TyrosinasewasthenaddedtotheAunanoclusterssolution,andasignificantdecreaseinfluorescenceintensitywasobserved,indicatingthefluorescencequenchingeffectofthenanoclusters.Thefluorescencequenchingwasfoundtobeconcentration-dependent,andthequenchingefficiencyincreasedwithincreasingtyrosinaseconcentration.Thequenchingmechanismwasfurtherinvestigatedbystudyingthefluorescencelifetimeofthenanoclusters. Discussion: ThefluorescencequenchingphenomenonobservedinthisstudycanbeattributedtotheeffectiveenergytransferbetweentheAunanoclustersandthetyrosinaseenzyme.Theinteractionbetweenthenanoclustersandthetyrosinasemoleculeleadstoadecreaseinfluorescenceintensityduetoanonradiativeenergytransferprocess.Thisprocesscanbefurtherexplore