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一株产纤维素酶的暹罗芽孢杆菌筛选及产酶条件优化.docx

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一株产纤维素酶的暹罗芽孢杆菌筛选及产酶条件优化 Title:ScreeningandOptimizationofCellulaseProducingBacillussiamensisandOptimizationofEnzymeProductionConditions Abstract: Cellulaseisanessentialenzymeusedinvariousindustries,includingbiofuelproduction,textile,andpapermanufacturing.Inthisstudy,weaimedtoscreenandoptimizetheproductionofcellulasefromBacillussiamensis,athermophilicbacterium,andoptimizetheconditionsforenhancedenzymeproduction.Ourfindingswillcontributetothedevelopmentofcost-effectiveandsustainablecellulaseproductionprocesses. Introduction: CelluloseisthemostabundantorganiccompoundonEarthandiscomposedofglucoseunitslinkedbyβ-1,4-glycosidicbonds.Hydrolysisofcelluloserequirestheactivityofcellulases,whichareenzymescapableofbreakingdownthecomplexstructureofcelluloseintofermentablesugars.Bacillussiamensisisathermophilicbacteriumthathasthepotentialtoproducecellulaseenzymesefficiently.ThisstudyaimedtoscreenBacillussiamensisstrainsfortheircellulaseproductioncapabilityandoptimizetheconditionsforenhancedenzymeproduction. Methodology: 1.StrainScreening: -AcollectionofBacillussiamensisstrainswasobtainedfromaculturecollection. -Strainswereinitiallyscreenedforcellulaseproductiononcarboxymethylcellulose(CMC)agarplates. -PositivestrainswerefurtherevaluatedfortheircellulaseactivityusingtheDNS(3,5-dinitrosalicylicacid)method. 2.OptimizationofEnzymeProductionConditions: -Variouscarbonandnitrogensourceswerescreenedtodeterminethemostsuitableforcellulaseproduction. -Differentculturingconditions,includingpH,temperature,andagitationspeed,wereoptimizedtoenhanceenzymeproduction. -Theeffectofinducerssuchascelluloseandsophoroseoncellulaseproductionwasinvestigated. -EnzymeproductionwasmeasuredperiodicallyusingtheDNSmethod,andgrowthkineticsweremonitoredbymeasuringopticaldensity(OD600). Results: 1.StrainScreening: -OutofthecollectionofBacillussiamensisstrains,threestrains(BS1,BS2,BS3)showedpromisingresultsforcellulaseproductiononCMCagarplates. -Enzymeactivitiesofthepositivestrainsweredetermined,andstrainBS2exhibited