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Box-Behnken响应面法优化辣木叶蛋白超声提取工艺研究.docx

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Box-Behnken响应面法优化辣木叶蛋白超声提取工艺研究 Title:OptimizationofUltrasonicExtractionProcessforMoringaLeafProteinusingBox-BehnkenResponseSurfaceMethodology Abstract: ThepresentstudyaimstooptimizetheultrasonicextractionprocessforMoringaleafproteinusingtheBox-Behnkenresponsesurfacemethodology(RSM).Moringaoleiferaisawell-knownmedicinalplantthatpossessesnumeroushealthbenefits.TheproteincontentinMoringaleavesisofsignificantimportance,asitishighlynutritiousandcanbeusedinvariousfoodandpharmaceuticalapplications.Ultrasonicextractionhasgainedattentioninrecentyearsduetoitsadvantagesofimprovedextractionefficiency,reducedprocessingtime,andavoidanceofhightemperatureandchemicalsolvents.Inthisstudy,weutilizetheBox-Behnkendesignofexperimentstodeterminetheoptimalcombinationofextractionparametersthatwillmaximizeproteinyield.Theindependentvariablesconsideredinthestudyareextractiontime,extractiontemperature,andsolvent-to-sampleratio.Thedependentvariableisproteinyield.Theexperimentalresultsarethenfittedtoasecond-orderpolynomialmodel,andtheoptimalextractionconditionsaredeterminedthroughresponsesurfaceanalysis.TheresultsofthisstudyprovidevaluableinsightsintotheextractionprocessofMoringaleafproteinandcanbeusedasareferenceforfutureresearchinthisdirection. Keywords:Moringaoleifera,ultrasonicextraction,Box-Behnkendesign,responsesurfacemethodology,proteinyield 1.Introduction: Moringaoleifera,commonlyknownasthedrumsticktreeorthehorseradishtree,isatropicalplantthathasgainedworldwideattentionduetoitsvarioushealthbenefits.TheleavesofMoringaoleiferaareknowntobearichsourceofproteins,vitamins,minerals,andantioxidants.Moringaleafproteinhasbeenreportedtopossessseveralhealth-promotingproperties,includinganti-inflammatory,antioxidant,andanticanceractivities.Therefore,theextractionandoptimizationofMoringaleafproteinisofsignificantinterestforboththefoodandpharmaceuticalindustries. 2.MaterialsandMethods: 2.1Materials:Moringaleaveswerecollected,cleaned,anddriedbeforeuse.Aproteinextractionsolvent,suchasphosphatebufferordis