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超声耦合双水相提取甘薯叶多酚的响应面优化.docx

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超声耦合双水相提取甘薯叶多酚的响应面优化 Introduction: Sweetpotatoleavesarearichsourceofpolyphenols,whichhavebeendemonstratedtopossessnumeroushealthbenefits.Theconventionalextractionofpolyphenolsfromsweetpotatoleavesinvolvessolventextraction,whichistime-consuming,labor-intensive,andoftenresultsinlowyieldsduetothepresenceofcomplexmatrixcomponents.Asanalternative,ultrasonic-assistedextraction(UAE)isgainingattentionasarapidandefficienttechniquefortheextractionofbioactivecompoundsfromvariousagriculturalwastes.TheaimofthisstudywastooptimizetheUAEconditionsfortheextractionofpolyphenolsfromsweetpotatoleavesusingaresponsesurfacemethodology(RSM). MaterialsandMethods: Materials: Sweetpotatoleaveswerecollectedfromalocalmarketinthecity.Chemicalsandsolventsusedwereofanalyticalgradeandpurchasedfromlocalsuppliers. Methods: Theextractionofpolyphenolsfromsweetpotatoleaveswasperformedbyultrasonic-assistedextractionusingatwo-phaseextractionsystemconsistingofethylacetateandwater.TheRSMwasusedtooptimizetheextractionconditions,includingextractiontime,ultrasonicpower,andsolvent-to-sampleratio.TheRSMwasdesignedusingacentralcompositedesign(CCD)withthreefactorsandfivelevels,resultingin20experimentalruns.TheextractedpolyphenolswerequantifiedbyusingtheFolin-Ciocalteumethod.ThedataobtainedwereanalyzedbyusingtheDesign-Expertsoftware. Results: TheresultsoftheRSMshowedthatallthreefactorshadasignificantinfluenceontheextractionyieldofpolyphenols.Thehighesttotalpolyphenolcontent(TPC)wasobtainedattheoptimalconditionsof40minextractiontime,225Wultrasonicpower,and30mL/gsolvent-to-sampleratio.ThepredictedoptimalTPCundertheseconditionswas35.57mg/g,whereastheexperimentalTPCwas35.62mg/g. Conclusion: ThisstudydemonstratedthatUAEwithatwo-phaseextractionsystemcouldeffectivelyextractpolyphenolsfromsweetpotatoleaves.TheRSManalysisrevealedthattheextractiontime,ultrasonicpower,andsolvent-to-sampleratiosignificantlyaffectedtheTPC,andtheoptimalconditionsforextractingpolyphenolswereobtained.Thesefindingsprovideabasisforthepromotionanddev