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两个CANON污水处理系统中氨氧化古菌的丰度和多样性研究.docx

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两个CANON污水处理系统中氨氧化古菌的丰度和多样性研究 Title:AbundanceandDiversityStudyofAmmonia-OxidizingArchaeainTwoCANONWastewaterTreatmentSystems Abstract: Thepresenceandactivityofammonia-oxidizingarchaea(AOA)playacrucialroleinthenitrogencycleofwastewatertreatmentsystems.Inthisstudy,weinvestigatedtheabundanceanddiversityofAOAintwoCANON(CompletelyAutotrophicNitrogen-removalOverNitrite)wastewatertreatmentsystems.TheaimwastodeepenourunderstandingoftheroleofAOAinnitrogenremovalandshedlightontheircommunitydynamicsinsuchsystems.OurfindingsrevealedtheimportanceofAOAaskeyplayersinnutrientremovalandprovidedinsightsintotheirecologicalfunctioning. 1.Introduction: Wastewatertreatmentplantsplayavitalroleinmaintainingwaterqualityandpreventingenvironmentalpollution.TheCANONprocessoffersanenergy-efficientandsustainableapproachtonitrogenremoval,utilizingnitriteasanintermediate.Ammoniaoxidation,thefirststepofnitrogenremoval,ispredominantlycarriedoutbyammonia-oxidizingbacteria(AOB)andammonia-oxidizingarchaea(AOA).WhileAOBhavebeenextensivelystudied,AOAhaverecentlyemergedasanimportantgroupofmicroorganismsinvolvedinnitrogencycling.ThisstudyaimstogaininsightsintotheabundanceanddiversityofAOAandtheirpotentialcontributionsinCANONwastewatertreatmentsystems. 2.Methods: TwoCANONwastewatertreatmentsystemslocatedindifferentregionswereselectedforthisstudy.Sampleswerecollectedatvariousstagesofthetreatmentprocess,includinginfluent,biofilm,activatedsludge,andeffluent.DNAextraction,PCRamplification,andsequencingoftheammoniamonooxygenasesubunitA(amoA)genewereperformedtoassesstheabundanceanddiversityofAOA.High-throughputsequencingtechnologiessuchasIlluminaMiSeqwereutilizedforampliconsequencing. 3.Results: ThesequencingresultsrevealeddiverseAOAcommunitiesinbothsystems.SeveralAOAgenera,includingNitrososphaera,Nitrosotalea,andNitrosopumilus,wereidentified.TherelativeabundanceofAOAvariedacrossdifferentstagesofthewastewatertreatmentprocess,withthehighestabundanceobservedinthebiofilmandactivatedsludgesamples.Thesefindingssuggestt