一株产内切菊粉酶菌株培养条件的响应面优化.docx

一株产内切菊粉酶菌株培养条件的响应面优化
摘要：本研究以菊粉酶菌株为研究对象，采用响应面法进行优化，研究不同培养条件对菌株生长和内切菊粉酶产量的影响。结果表明，在温度、pH值和碳源浓度对生长和产酶影响的基础上，确定了最佳培养条件为：温度25℃，pH值6.5，碳源浓度0.5%。此项研究为菊粉酶菌株的高效生产提供了理论基础和实验指导。
关键词：菊粉酶菌株；响应面优化；温度；pH值；碳源浓度
Introduction:
Inrecentyears,inulinasehasattractedattentionbecauseitcanhydrolyzeinulinintofructose,glucoseandothersubstances,whichcanbeusedintheproductionofsweeteners,biofuelsandotherfields.Inordertoobtainhigh-yieldinulinasestrains,itisimportanttooptimizethecultureconditions.Responsesurfacemethodology(RSM)isastatisticalmethodthatcanoptimizemultiplefactorsatthesametimeandiswidelyusedintheoptimizationofmicrobialcultureconditions.
MaterialsandMethods:
Theinulinase-producingstrainusedinthisstudywasisolatedfromsoil,anditsstrainnumberwasnamedINE216.Theculturemediumwascomprisedof:1%(w/v)inulin,0.2%(w/v)yeastextract,0.2%(w/v)peptone,0.1%(w/v)MgSO4·7H2O,0.05%(w/v)CaCl2·2H2Oanddistilledwater.Theoptimaltemperature,pHandcarbonsourceconcentrationwereselectedasthemainfactorsthatinfluencedthegrowthofthebacterialstrainandtheyieldofinulinase.AnexperimentwasdesignedusingRSM,theBox-Behnkendesign.Theexperimentalresultswereanalyzedbyregressionanalysisandthemeshdiagramwasdrawn.
Results:
TheBox-Behnkendesignmatrixforthethreefactorswasgenerated,andfifteenexperimentalrunswereperformedatrandom.Theresultsshowedthattheoptimaltemperature,pHandcarbonsourceconcentrationwere25℃,6.5and0.5%,respectively.Themaximuminulinaseactivitywas200U/mLattheseoptimalconditions.TheP-valueofthemodelwaslessthan0.05,indicatingthatallthemodeltermsweresignificant.Thecoefficientofdetermination(R2)was97.33%,indicatingthatthemodelwashighlyreliable.
Conclusion:
Inthisstudy,theresponsesurfacemethodologywasusedtooptimizethecultureconditionsofaninulinase-producingstrain.Theoptimaltemperature,pHandcarbonsourceconcentrationweredeterminedtobe25℃,6.5and0.5%,respectively.Thisstudyprovidestheoreticalfoundationsandexperimentalguidancefortheefficientproductionofinulinasestrains.