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高纯度大豆染料木苷的制备工艺研究 Title:StudyonthePreparationProcessofHigh-PuritySoybeanDyeGenistein Abstract: Thispaperpresentsacomprehensivestudyonthepreparationprocessofhigh-puritysoybeandyegenistein.Genistein,anaturalcompoundfoundinsoybeans,hasgainedsignificantattentioninrecentyearsduetoitspotentialapplicationsinvariousfields,includingtextiledyeing.Theaimofthisstudyistoexplorethemostefficientandenvironmentallyfriendlymethodforextractingandpurifyinggenisteinfromsoybeans.Thepaperdiscussestheextractionofgenistein,itspurificationtechniques,andthecharacterizationoftheobtainedhigh-puritygenistein.Ultimately,thisresearchcontributestothedevelopmentofsustainableandcost-effectivemethodsforproducingsoybean-baseddyes. 1.Introduction Genisteinisanaturalisoflavonecompoundfoundinsoybeans,whichhasattractedconsiderableinterestduetoitsuniquechemicalstructureandpotentialapplicationsasatextiledye.Theproductionofhigh-puritygenisteinfromsoybeanscanprovideasustainablesourceofdyeandreducetherelianceonsyntheticdyes,whichoftenhavenegativeenvironmentalimpacts.Thispaperfocusesonexaminingthedifferentstagesinvolvedinthepreparationofhigh-puritygenisteinfromsoybeans. 2.ExtractionofGenistein Theextractionofgenisteinfromsoybeansisthefirstcrucialstepinthepreparationprocess.Variousmethods,suchassolventextraction,supercriticalfluidextraction,andultrasound-assistedextraction,havebeenexploredtoachieveefficientextractionwithhighgenisteinyield.Theadvantagesanddrawbacksofeachmethodarediscussed,consideringfactorssuchasextractiontime,temperature,solventtype,andgenisteinconcentration. 3.PurificationTechniques Afterextraction,crudegenisteinneedstobepurifiedtoenhanceitspurityandremoveimpurities,suchasproteins,carbohydrates,andpigments.Severalpurificationtechniques,includingsolventprecipitation,chromatography,andmembranefiltration,areexaminedinthisstudy.Theeffectivenessofeachmethodintermsofgenisteinrecovery,purity,andenvironmentalimpactisassessed. 4.CharacterizationofHigh-PurityGenistein Theobtainedhigh-puritygenisteinischara