产漆酶裂褶菌的筛选及其产酶培养条件优化研究.docx

产漆酶裂褶菌的筛选及其产酶培养条件优化研究摘要：从采自秦岭的森林腐木菌苔上分离到一株产漆酶的裂褶菌（SchizophyllumcommuneFr.）菌株mys005对其产漆酶培养方式、培养基组成及培养条件进行了优化试验。结果表明裂褶菌菌株mys005的产漆酶适宜培养方式为液体浅层振荡培养含有Cu2+、Mn2+、Zn2+的微量元素复合液、洋葱（AlliumcepaL.）、麦（TriticumaestivumL.）麸对其产漆酶都有较大的促进作用而愈创木酚则对其生长和产漆酶有抑制作用。由新鲜马铃薯（SolanumtuberosumL.）200g、K2HPO41.00g、NaCl0.50g、MgSO4·7H2O0.50g、NaNO32.50g、CaCl2·2H2O0.10g、FeCl30.02g、吐温-801.20mL溶于1000mL去离子水中组成的培养基在添加微量元素复合液（50mg/mLCuCl2·2H2O+20mg/mLMnSO4·H2O+10mg/mLZnSO4·7H2O）3mL、洋葱100g、麦麸10g后并且培养基起始pH4.5、培养温度30℃、培养摇床转速200r/min、培养时间7d则摇瓶发酵的漆酶产酶量最高可达1121U/mL显示出了良好的工业开发潜力。关键词：漆酶；裂褶菌（SchizophyllumcommuneFr.）；筛选；产酶培养基；优化中图分类号：Q554+.9；S646.1+9；Q93-33文献标识码：A文章编号：0439-8114（2013）12-2792-06IsolationofSchizophyllumcommuneProducingLaccaseandOptimizationofLaccaseProductionConditionsZHAOWen-juanXUSheng-yunRENPing（EnzymeEngineeringInstituteofShaanxiSciences/ShaanxiEngineeringCenterforEnzymeTechnologyXi’an710600China）Abstract：ASchizophyllumcommuneFr.strainmys005producinglaccasewasisolatedfromtherottenwoodlawninforestofQinlin.Theculturemethodforlaccaseproductionmediumcompositionandcultivationconditionswasoptimized.ResultsshowedthatthesuitablecultivationmethodforS.communestrainmys005toproducelaccasewasshallowliquidshakingculture.TraceelementscompoundliquidcontainingCu2+Mn2+Zn2+onion（AlliumcepaL.）wheat（TriticumaestivumL.）brancouldpromotelaccaseproduction；whileguaiacolcouldinhibitthegrowthandlaccaseproduction.Ifaddingtraceelementcompoundsolution（50mg/mLCuCl2·2H2O+20mg/mLMnSO4·H2O+10mg/mLZnSO4·7H2O）3mLonion100gwheatbran10ginthemediumcontainingfreshpotato（SolanumtuberosumL.）200gK2HPO41.00gNaCl0.50gMgSO4·7H2O0.50gNaNO32.50gCaCl2·2H2O0.10g、FeCl30.02g、tween-801.20mLin1000mLdeionizedwaterandkeepingthestartingpHat4.5startingtemperatureat30℃cultureshakerspee