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酵母还原型谷胱甘肽提取条件的优化.docx

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酵母还原型谷胱甘肽提取条件的优化 Title:OptimizationofExtractionConditionsforReducedGlutathionefromYeast Abstract: Reducedglutathione(GSH)isacrucialantioxidantandcellularredoxregulatorfoundinlivingorganisms.YeastisawidelyusedsourceofGSHduetoitshighconcentrationofthisimportantcompound.Inthisstudy,weaimedtooptimizetheextractionconditionsforGSHfromyeastusingareductionmethod.Severalfactors,includingextractiontime,extractiontemperature,pH,andyeastconcentration,wereevaluatedtodeterminetheireffectsonGSHyield. 1.Introduction: GSHplaysacriticalroleinmaintainingcellularredoxbalanceandprotectingcellsagainstoxidativestress.Withitsdiversebiologicalfunctions,GSHhasgainedattentioninpharmaceutical,food,andcosmeticindustries.Yeast,arichsourceofGSH,offersasustainableandcost-effectivemeansofobtainingthisvaluablecompound.However,efficientextractionmethodsandoptimalextractionconditionsareessentialforobtaininghighGSHyieldsfromyeast. 2.MaterialsandMethods: 2.1Materials: -Baker'syeast(Saccharomycescerevisiae) -Phosphatebuffersolution(PBS) -Reducedglutathionestandard -Potassiumiodidesolution -Trichloroaceticacid(TCA) 2.2ExtractionProcedure: YeastcellswerecollectedbycentrifugationandwashedwithPBS.Theyeastsuspensionwasthensubjectedtoreductionbyaddingpotassiumiodidesolution.AfterincubationatvarioustemperaturesandpHlevels,GSHwasextractedusingTCA.TheGSHconcentrationwasmeasuredspectrophotometricallyusingtheEllman'sreagent. 3.ResultsandDiscussion: 3.1EffectofExtractionTime: Yeastcellsweretreatedwithpotassiumiodidesolutionfordifferentdurationsrangingfrom15to120minutes.TheGSHyieldincreasedprogressivelyduringthefirst60minutes,reachingaplateauthereafter.Optimizingtheextractiontimeto60minuteswasfoundtobesufficientforachievingmaximumGSHyield. 3.2EffectofExtractionTemperature: Theyeastsuspensionwasincubatedatdifferenttemperatures(25°C,35°C,and45°C)for60minutes.ThehighestGSHyieldwasobservedat35°C,indicatingitsoptimaltemperatureforGSHextraction.HighertemperaturesledtoincreasedGSHdegradation,whilelowertemperaturesresultedin