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脂溶性荧光染料测定微生物油脂的研究进展.docx

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脂溶性荧光染料测定微生物油脂的研究进展 Title:ResearchProgressontheUseofLipophilicFluorescentDyesforMicrobialLipidAnalysis Abstract: Microbiallipids,mainlyintheformoftriglycerides,playavitalroleinthebioconversionofvariouscarbonsourcesintovaluablebiofuelsandbiochemicals.Quantitativeanalysisofmicrobiallipidsiscrucialforunderstandingtheirmetabolismandoptimizinglipidproductionprocesses.Traditionalmethodsforlipidanalysis,suchasgaschromatography,liquidchromatography,andmassspectrometry,aretime-consuming,labor-intensive,andoftenrequirecomplexsamplepreparationprocedures.Inrecentyears,theuseoflipophilicfluorescentdyesasanalternativemethodforrapidandconvenientlipidanalysishasattractedincreasingattention.Thispaperreviewstheresearchprogressintheapplicationoflipophilicfluorescentdyesforthedeterminationofmicrobiallipids,focusingontheiradvantages,limitations,andfutureprospects. 1.Introduction Microbiallipidsareconsideredapromisingrenewableresourceduetotheirpotentialuseasbiofuelsandbiochemicals.Toexploretheirfullpotential,itisnecessarytodevelopefficientandreliablemethodsforassessinglipidproductivityandcomposition.Traditionalmethodsforlipidanalysishavelimitations,highlightingtheneedforalternativeapproachessuchaslipophilicfluorescentdyes. 2.PrinciplesofLipophilicFluorescentDye-basedLipidAnalysis Thebasicprincipleoflipophilicfluorescentdye-basedlipidanalysisliesintheselectiveaffinityofthedyesforlipidmolecules.Thesedyespossessnon-polarstructuresthatallowthemtoeasilydissolveinlipids.Uponbindingtolipids,theyundergoamolecularchangethatleadstofluorescenceemission.Theintensityoffluorescenceisdirectlyproportionaltothequantityoflipids,enablingrapidandquantitativemeasurement. 3.AdvantagesofLipophilicFluorescentDye-basedLipidAnalysis 3.1Rapidanalysis:Comparedtotraditionalmethods,lipophilicfluorescentdye-basedlipidanalysisofferssignificantlyreducedanalysistime,makingitidealforhigh-throughputscreeningandmonitoring. 3.2Sensitivity:Lipophilicfluorescentdyesdemonstratehighsensitivity,enablingthedetectionoflipidsatlowco