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艾叶水提氯仿部位抑菌活性及其作用机制研究.docx

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艾叶水提氯仿部位抑菌活性及其作用机制研究 Title:AntibacterialActivitiesandMechanismofChloroformExtractfromArtemisiaargyiLeaves Abstract: Artemisiaargyi,commonlyknownasAiYe,hasbeenwidelyusedintraditionalmedicineduetoitsnumerouspharmacologicalproperties,includingantimicrobialactivities.Inthisstudy,weinvestigatedtheantibacterialactivitiesofthechloroformextractfromArtemisiaargyileavesandexploreditsunderlyingmechanisms.Theresultsdemonstratethatthechloroformextractexhibitedsignificantantibacterialeffectsagainstarangeofpathogenicbacteria,indicatingitspotentialasanaturalantibacterialagent.Furthermore,weelucidatedtheactionmechanismofthechloroformextract,revealingitsabilitytodisruptbacterialcellmembraneintegrityandinhibitimportantintracellularprocesses.ThesefindingsprovidevaluableinsightsintotheantimicrobialpropertiesofArtemisiaargyiandhighlightitspotentialforthedevelopmentofnewantimicrobialagents. 1.Introduction Inrecentyears,theincreasingemergenceofantibiotic-resistantbacteriahasposedasignificantthreattoglobalhealth.Therefore,thesearchforalternativeantimicrobialagentsbecomesimperative.TraditionalChinesemedicineprovidesarichsourceofpotentialantimicrobialcandidates.Artemisiaargyi,awell-knownherbalplantinChina,hasgainedattentionduetoitsdiversebiologicalactivities,includingantimicrobialproperties.Inthisstudy,weaimedtoinvestigatetheantibacterialactivitiesofthechloroformextractfromArtemisiaargyileavesandelucidateitsmechanismofaction. 2.MaterialsandMethods 2.1Plantmaterialandpreparationofthechloroformextract Artemisiaargyileaveswerecollected,dried,andgroundintoafinepowder.ThechloroformextractwasobtainedusingaSoxhletextractor.Theextractwasthenconcentratedandstoredforfurtheranalysis. 2.2Antibacterialactivityassay Theantibacterialactivitiesofthechloroformextractweredeterminedusingtheagardiffusionmethod.Variouspathogenicbacterialstrainswereculturedandincubatedwithdifferentconcentrationsofthechloroformextract.Theinhibitionzonesweremeasuredtoassesstheantibacterialeffects. 2.3Mechanismstudies Toinvestigateth