均匀设计优化碱法提取银杏蛋白工艺.docx

均匀设计优化碱法提取银杏蛋白工艺
Title:OptimizationofAlkalineExtractionProcessforGinkgoBilobaProteinbyUniformDesign
Abstract:
Thealkalineextractionprocessisacommonlyusedmethodforisolatingproteinfromplant-basedmaterials.Inthisstudy,weaimedtooptimizethealkalineextractionprocessforGinkgobilobaproteinusingtheuniformdesignapproach.Aseriesofexperimentswereconductedtoinvestigatetheeffectsofkeyprocessparameters,includingalkaliconcentration,extractiontime,andliquid-to-solidratio,ontheproteinextractionyield.Theresultsrevealedsignificantinteractionsamongtheseprocessvariables,andtheoptimalconditionsformaximumproteinyieldweredetermined.ThisresearchprovidesvaluableinsightsfortheefficientextractionofGinkgobilobaproteinandestablishesafoundationforitsapplicationinvariousfields.
Introduction:
Ginkgobiloba,awidelyrecognizedandvaluedmedicinalplant,isknownforitsvarioushealthbenefits.Ginkgobilobaprotein,asoneofitsprimarybioactivecomponents,hasbeenextensivelystudiedduetoitspotentialtherapeuticproperties.Alkalineextractionisapreferredmethodforisolatingproteinsfromplantsourcesasitoffersseveraladvantages,includingsimplicity,cost-effectiveness,andwideapplicability.Therefore,theoptimizationofthealkalineextractionprocessforGinkgobilobaproteinisofgreatsignificancetoenhanceitspotentialapplications.
Methods:
Uniformdesign,anefficientstatisticalexperimentaldesignmethod,wasemployedinthisstudytooptimizethealkalineextractionprocessforGinkgobilobaprotein.Toevaluatetheeffectsofimportantprocessparameters,includingalkaliconcentration,extractiontime,andliquid-to-solidratio,auniformdesignmatrixwasconstructed.Basedonthematrix,aseriesofexperimentswereperformed,andtheproteinextractionyieldsweremeasured.Theobtaineddatawasthenanalyzedusingresponsesurfacemethodology(RSM)todeterminetheoptimumconditionsformaximumproteinyield.
ResultsandDiscussion:
Theexperimentsrevealedthatalkaliconcentration,extractiontime,andliquid-to-solidratioallsignificantlyinfluencedtheproteinextractionyield.Additionally,therewereconsiderableinteractionsamon