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嗜盐石油烃降解菌的筛选及原位修复效果研究（英文）.docx

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嗜盐石油烃降解菌的筛选及原位修复效果研究（英文） ScreeningofHalophilicPetroleumHydrocarbon-DegradingBacteriaandInSituRemediationEffectResearch Introduction Petroleumhydrocarbonpollutionisaglobalenvironmentalissuethathasattractedwidespreadattention.Thevariousproblemscausedbythiscontaminationhavespurredresearchintoeffectivesolutions.Bioremediationisoneofthemostpracticalandeco-friendlymethodsavailable,relyingontheabilityofmicroorganismstodegradehydrocarboncompounds.Inthisstudy,wefocusedonthescreeningofhalophilicpetroleumhydrocarbon-degradingbacteriaandtheirremediationeffectsinsitu. Methods Thehalophilicpetroleumhydrocarbon-degradingbacteriawerefirstscreenedfromsalt-tolerantoilfieldsoilsbyusingasalt-tolerantmedium.Thestrainswereidentifiedbasedontheirmorphology,physiologicalandbiochemicalcharacteristics,and16SrDNAsequenceanalysis.Toevaluatethedegradationabilityofthestrains,weconductedaseriesofexperimentsusingvariouspetroleumhydrocarboncompounds(C10-C30).Thedegradationratewasmeasuredovertimeusinggaschromatography. Oncethestrainsweresuccessfullyidentified,weconductedaninsituremediationexperimentatapetroleum-contaminatedsiteusingamicrobialdegradationagent.Forcomparison,wealsoconductedabiostimulationremediationexperimentbyaddingnutrientstothecontaminatedsoil.Thepetroleumhydrocarbonconcentrationandbacterialabundanceweremeasuredatsetintervalsduringtheremediationprocesstoevaluatetheeffectivenessofeachmethod. Results Fivehalophilicpetroleumhydrocarbon-degradingbacterialstrainsweresuccessfullyisolatedandidentified,whichincludedParacoccushalophilus,Halomonaspantelleriensis,Planococcusrifietoensis,Bacilluslicheniformis,andBacillussubtilis.Allofthestrainsshowedhighdegradationratesforvarioushydrocarbons,with80-90%degradationachievedwithin15days. Intheinsituremediationexperiment,themicrobialdegradationagentshowedasuperioreffectincomparisonwithbiostimulation.Thedegradationefficiencyoftotalpetroleumhydrocarbon(TPH)bythemicrobialdegradationagentwas85.6%,whileonly64.7%wasachievedbybiostimulationafteraperiodof60d