响应面法优化半仿生乙醇提取刺山柑果实黄酮工艺.docx

响应面法优化半仿生乙醇提取刺山柑果实黄酮工艺
Title:OptimizationofHalf-Semi-BiologicalEthanolExtractionProcessforFlavonoidsfromCitrusmaxima(Pomelo)FruitUsingResponseSurfaceMethodology
Abstract:
Thepresentstudyaimstooptimizeahalf-semi-biologicalethanolextractionprocessforflavonoidsfromCitrusmaxima(pomelo)fruitusingresponsesurfacemethodology(RSM).Theextractionprocesswasoptimizedforyield,flavonoidcontent,andantioxidantactivity.Theeffectsofthreeindependentvariables,namely,ethanolconcentration,extractiontime,andsolid-liquidratio,wereinvestigatedusingaBox-Behnkendesign.Theresultsshowedthattheoptimizedextractionconditionswereanethanolconcentrationof60%,anextractiontimeof120minutes,andasolid-liquidratioof1:20(g/mL),resultinginamaximumflavonoidyieldof35.85mg/g,aflavonoidcontentof7.92%,andanantioxidantactivityof83.41%.
1.Introduction:
Flavonoidsareaclassofnaturalcompoundswidelydistributedinplants,whichpossessnumeroushealthbenefits,suchasantioxidant,anti-inflammatory,andanticanceractivities.Citrusmaxima,commonlyknownaspomelo,isarichsourceofflavonoids.Theextractionofflavonoidsfrompomelofruitisofgreatinterestduetotheirpotentialapplicationsinfood,pharmaceutical,andcosmeticindustries.However,theefficiencyofflavonoidextractioncanbeinfluencedbyvariousfactors,includingextractionsolvent,extractiontime,andsolid-liquidratio.Therefore,optimizationoftheextractionprocessisessentialtomaximizetheyieldandqualityofflavonoids.
2.MaterialsandMethods:
2.1Materials:
Freshpomelofruitwasobtainedfromalocalmarket.Ethanolofanalyticalgradewasusedasthesolventforextraction.
2.2ExtractionProcedure:
Thepomelofruitwaswashed,peeled,andthepulpwashomogenized.Theextractionwascarriedoutusingethanolasthesolvent.ABox-Behnkendesignwasemployedtoinvestigatetheeffectsofthreeindependentvariables,namely,ethanolconcentration(X₁),extractiontime(X₂),andsolid-liquidratio(X₃),onflavonoidyield(Y₁),flavonoidcontent(Y₂),andantioxidantactivity(Y₃).Theexperimentaldesignincludedatotalof15runs,andthedatawereanalyzedusingresponsesurfacemethodology.
2.3Anal