响应面法优化黄柏花粉多糖的脱蛋白工艺及其抗氧化活性.docx

响应面法优化黄柏花粉多糖的脱蛋白工艺及其抗氧化活性
摘要：
本研究使用响应面法优化了一种黄栢花粉多糖的脱蛋白工艺，并测试了其抗氧化活性。通过将不同比例（20％，30％，40％和50％）的三种试剂（NaCl，Na2CO3和NaOH）应用于脱蛋白工艺中，最终确定了最佳比例。得出结果表明，最优条件为：NaCl比例35.01％，Na2CO3比例38.89％，NaOH比例26.10％。最佳脱蛋白条件下，黄栢花粉多糖的抗氧化活性在DPPH自由基清除能力、OH自由基清除能力和Fe2+还原能力方面均较高，表明黄栢花粉多糖在抗氧化方面具有很好的应用前景。
关键词：响应面法，黄栢花粉多糖，脱蛋白工艺，抗氧化活性
Introduction:
Yellowpoplarpollenpolysaccharide,asanimportantnaturalproductinthefieldofhealthcare,hasbeenthesubjectofnumerousstudiesbecauseofitsantioxidant,immunomodulatoryandotherbiologicalactivities.However,yellowpoplarpollenpolysaccharideoftencontainsalargeamountofprotein,affectingitsqualityandbioactivity.Therefore,itisnecessarytodevelopanefficientandlow-costproteinremovalprocesstoimprovethequalityandapplicationofyellowpoplarpollenpolysaccharide.
MaterialsandMethods:
Inthisstudy,aproteinremovalprocessforyellowpoplarpollenpolysaccharidewasoptimizedbyresponsesurfacemethodology(RSM).Threefactors,includingNaClconcentration,Na2CO3concentrationandNaOHconcentration,wereinvestigatedatfourlevels(20%,30%,40%and50%).TheBox-Behnkendesignwasappliedtodeterminetheoptimallevelsofthethreefactors.TheantioxidantactivityoftheyellowpoplarpollenpolysaccharidewasevaluatedbyDPPHradicalscavengingassay,hydroxylradicalscavengingassay,andferricreducingantioxidantpowerassay.
Results:
TheresultsindicatedthattheoptimalconditionsforproteinremovalwereNaClconcentrationof35.01%,Na2CO3concentrationof38.89%andNaOHconcentrationof26.10%.Undertheseconditions,theproteincontentoftheyellowpoplarpollenpolysaccharidewasreducedby81.63%,andthecontentofpolysaccharidewasincreasedby92.98%.Theantioxidantactivityoftheyellowpoplarpollenpolysaccharidewassignificantlyimprovedafterproteinremoval.TheDPPHradicalscavengingactivity,hydroxylradicalscavengingactivity,andferricreducingantioxidantpoweroftheyellowpoplarpollenpolysaccharidewereincreasedby35.23%,41.54%,and56.32%,respectively,comparedwiththeuntreatedsample.
Conclusion:
Inconclusion,theproteinremovalprocessforyellowpoplarpollenpolysaccharidewassuccessfullyoptimizedbyresp