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Wdr1基因敲除对成年小鼠心脏的影响研究.docx

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Wdr1基因敲除对成年小鼠心脏的影响研究 Title:TheImpactofWdr1GeneKnockoutontheAdultMouseHeart:AStudy Introduction: TheWdr1gene,alsoknownascoronin-1C,isamemberofthecoroninfamilyofactin-bindingproteins.Itplaysacrucialroleinregulatingactindynamicsandisinvolvedinvariouscellularprocesses,includingcellmigration,celldivision,andcellshapechanges.PreviousstudieshavesuggestedthatWdr1isalsoimportantincardiovasculardevelopmentandhomeostasis.However,thespecificimpactofWdr1geneknockoutontheadultmouseheartremainslargelyunknown.ThisstudyaimstoinvestigatetheconsequencesofWdr1geneknockoutontheadultmouseheart,focusingoncardiacstructure,function,andgeneexpressionprofiling. Methods: 1.Wdr1geneknockoutmicegeneration:Wdr1geneknockoutmicewillbegeneratedusingCRISPR-Cas9technologyorgenetargetingmethods.Acontrolgroupofwild-typemicewillalsobeincluded. 2.Cardiacstructureandfunctionassessment:Echocardiographywillbeperformedtoevaluatecardiacstructureandfunctionparameters,includingfractionalshortening,ejectionfraction,leftventricularmass,anddiastolicandsystolicfunction. 3.Histologicalanalysis:Hearttissueswillbecollectedandsubjectedtohematoxylinandeosin(H&E)stainingtoassesshistologicalchanges,includingcollagendeposition,fibrosis,andinflammation. 4.Geneexpressionprofiling:RNAwillbeextractedfromhearttissues,andgeneexpressionwillbeanalyzedusingatranscriptomeanalysisapproach,suchasRNAsequencing.Differentiallyexpressedgenesbetweentheknockoutandcontrolgroupswillbeidentifiedandfunctionallyannotated. 5.Westernblotting:Proteinexpressionlevelsofkeycardiacmarkers,suchasTroponinIandMyosinHeavyChain,willbeassessedusingwesternblotting. Results: 1.Cardiacstructureandfunction:ItisanticipatedthatWdr1geneknockoutwillleadtoalterationsincardiacstructureandfunctionparameters,asevidencedbyreducedfractionalshorteningandejectionfraction,increasedleftventricularmass,andimpaireddiastolicandsystolicfunction. 2.Histologicalanalysis:Wdr1geneknockoutmicemayexhibitcardiachistopathologicalchanges,includingincreasedcollagendeposition,fibrosis,andinf