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采后枇杷果实冷害木质化相关PRX成员鉴别及其转录调控研究.docx

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采后枇杷果实冷害木质化相关PRX成员鉴别及其转录调控研究 Title:IdentificationofPostharvestColdInjuryandStudyofTranscriptionalRegulationinWoodLignificationofLoquat(Eriobotryajaponica)Fruit Abstract: Postharvestcoldinjuryisasignificantissueinloquat(Eriobotryajaponica)fruit,leadingtochillinginjury,lossofquality,andincreasedsusceptibilitytodecay.Woodlignificationisanessentialprocessinplantsandplaysacrucialroleinprotectingagainstadverseenvironmentalconditions.Inthisstudy,weaimedtoidentifythemembersofthePRXgenefamilyinvolvedinpostharvestcoldinjuryinloquatfruitandinvestigatethetranscriptionalregulationofwoodlignificationinresponsetocoldstress. 1.Introduction: Postharvestcoldinjuryisacommonprobleminmanyfruitcrops,includingloquat.Chillinginjury,characterizedbytissuediscoloration,pitting,andaccelerateddecay,greatlyreducesthequalityandmarketvalueoffruit.Understandingthemolecularmechanismsunderlyingpostharvestcoldinjuryandwoodlignificationiscrucialfordevelopingstrategiestoimprovefruitqualityandstoragelife. 2.IdentificationandCharacterizationofPRXMembers: Theperoxidase(PRX)genefamilyplaysavitalroleinplantresponsetovariousabioticstresses.Inthisstudy,weperformedacomprehensiveanalysisofthePRXgenefamilyinloquatfruit.Byminingtheloquatgenomedatabase,weidentifiedatotalofXPRXfamilymembers,whichwerefurthercharacterizedbasedontheirphylogeneticrelationships,conserveddomains,andgeneexpressionpatterns. 3.AnalysisofPRXGeneExpressionduringColdStorage: ToinvestigatetheinvolvementofPRXgenesinpostharvestcoldinjury,weassessedtheirexpressionpatternsduringcoldstorageatdifferenttimepoints.UsingqRT-PCR,wefoundthatseveralPRXgenesweresignificantlyupregulateduponcoldexposure,suggestingtheirpotentialroleincoldtolerance.FurtherfunctionalanalysisusingtransgenicplantswithoverexpressedorsilencedPRXgeneswillhelpconfirmtheirinvolvementinpostharvestcoldinjury. 4.TranscriptionalRegulationofWoodLignification: Woodlignificationisacomplexprocessinvolvingtheactivationofvarioustranscriptionfactorsandenzymes.Toelucidatethetranscriptionalregulatio