预览加载中，请您耐心等待几秒...

草酸青霉Htf和StuA调控孢子生成与糖苷水解酶表达合成的功能研究.docx

预览
1/2
2/2

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

草酸青霉Htf和StuA调控孢子生成与糖苷水解酶表达合成的功能研究 Title:FunctionalCharacterizationofOxalatePenicilliumHtfandStuAinSporeFormationandGlycosideHydrolaseExpression Abstract: Fungalsporeformationandtheexpressionofglycosidehydrolaseareessentialprocessesinthelifecycleoffilamentousfungi.Theregulatorymechanismsunderlyingtheseprocessesarenotfullyunderstood,especiallyinthecaseoftheoxalate-producingfungusPenicillium.Inthisstudy,weinvestigatedthefunctionalrolesoftwotranscriptionfactors,HtfandStuA,insporeformationandglycosidehydrolaseexpressioninPenicilliumoxalatum.Ourfindingsprovideinsightsintothecomplexregulatorypathwaysinvolvedinfungaldevelopmentandsecondarymetabolism. Introduction: Filamentousfungiareimportantproducersofvaluablebioactivecompounds,andtheirlifecyclesincludedifferentiationintospecializedcelltypessuchasspores.Sporeformationisregulatedbyacomplexnetworkofgeneticfactorsthatcoordinatedevelopmentalprocesses.Additionally,filamentousfungiproduceavarietyofglycosidehydrolasesthatareinvolvedinthedegradationofcomplexcarbohydrates.Theexpressionofglycosidehydrolasesistightlyregulatedandoftenassociatedwiththepresenceofspecificinducingsubstrates.Understandingtheregulationofsporeformationandglycosidehydrolaseexpressioniscrucialforbothfundamentalresearchandbiotechnologicalapplications.WhilesomeregulatoryfactorshavebeencharacterizedinmodelfungisuchasAspergillusandNeurospora,thespecificrolesofHtfandStuAinPenicilliumoxalatumremainlargelyunknown. Methods: ToinvestigatethefunctionalrolesofHtfandStuAinsporeformationandglycosidehydrolaseexpression,weemployedacombinationofgeneticmanipulation,transcriptomicanalysis,andphenotypicassays.HtfandStuAdeletionmutantsweregeneratedusinghomologousrecombination,andtheirphenotypeswerecomparedtothewild-typestrain.Transcriptomicanalysiswasperformedtoidentifydifferentiallyregulatedgenesinthemutantscomparedtothewildtype.Phenotypicassayswereusedtoassesssporeformationandglycosidehydrolaseactivity. ResultsandDiscussion: OurresultsrevealedthatthedeletionofHtfandStuAhadprofo