肢体远程缺血后处理对兔脊髓缺血损伤的保护作用及机制研究.docx

肢体远程缺血后处理对兔脊髓缺血损伤的保护作用及机制研究
肢体远程缺血后处理对兔脊髓缺血损伤的保护作用及机制研究
摘要：肢体远程缺血后处理（postconditioning）是一种新颖的保护性策略。本文旨在探究肢体远程缺血后处理对兔脊髓缺血损伤的保护作用及机制。采用36只新西兰兔，分为缺血组（n=16）和肢体远程缺血后处理组（n=20）。设计缺血模型后，对处理组进行肢体远程缺血后处理，比较两组动物的神经功能缺失程度、组织损伤评分和激活细胞凋亡相关蛋白的表达情况。结果显示，肢体远程缺血后处理组的神经功能缺失程度显著低于缺血组(P<0.05)，同时组织损伤评分也较低，证明可改善脊髓缺血后的组织损伤。在细胞水平上，经过处理的组织中细胞凋亡相关蛋白如Caspase-3、Bax表达量均显著低于处理前的组织(P<0.05)，同时Bcl-2的表达量上升，表明肢体远程缺血后处理能有效抑制神经细胞凋亡过程。
综上，肢体远程缺血后处理可通过抑制神经细胞凋亡过程，保护兔脊髓免受缺血-再灌注后的损伤。
关键词：肢体远程缺血后处理，脊髓缺血损伤，神经功能缺失程度，组织损伤评分，凋亡相关蛋白
Abstract:Limbremotepostconditioningisanovelprotectivestrategy.Theaimofthisstudywastoinvestigatetheprotectiveeffectandmechanismoflimbremotepostconditioningonspinalcordischemicinjuryinrabbits.36NewZealandrabbitsweredividedintoischemiagroup(n=16)andlimbremotepostconditioninggroup(n=20).Aftertheischemicmodelwasdesigned,limbremotepostconditioningwasperformedonthetreatmentgroup.Thedegreeofneurologicalfunctionloss,tissuedamagescore,andexpressionofapoptosis-relatedproteinswerecomparedbetweenthetwogroups.
Theresultsshowedthatthedegreeofneurologicalfunctionlossinthelimbremotepostconditioninggroupwassignificantlylowerthanthatintheischemiagroup(P<0.05).Atthesametime,thetissuedamagescorewasalsolower,indicatinganimprovementintissuedamageafterspinalcordischemia.Atthecellularlevel,theexpressionofapoptosis-relatedproteinssuchasCaspase-3andBaxinthetreatedtissuewassignificantlylowerthanthatinuntreatedtissue(P<0.05).Meanwhile,theexpressionofBcl-2increased,indicatingthatlimbremotepostconditioningcaneffectivelyinhibittheprocessofneuronalapoptosis.
Inconclusion,limbremotepostconditioningcanprotectrabbitsfromspinalcordischemicinjurybyinhibitingtheprocessofneuronalapoptosis.
Keywords:limbremotepostconditioning,spinalcordischemicinjury,degreeofneurologicalfunctionloss,tissuedamagescore,apoptosis-relatedproteins.