脂联素预处理对大鼠肝脏缺血再灌注胆管损伤的保护作用研究.docx

脂联素预处理对大鼠肝脏缺血再灌注胆管损伤的保护作用研究
摘要
目的：探讨脂联素预处理对大鼠肝脏缺血再灌注胆管损伤的保护作用。
方法：将50只雄性SD大鼠随机分为5组（n=10），正常组、缺血再灌注组、脂联素组、脂联素-缺血再灌注组、缺血再灌注-脂联素组。缺血再灌注组、脂联素-缺血再灌注组、缺血再灌注-脂联素组均进行肝门阻断20分钟再灌注60分钟；脂联素组和脂联素-缺血再灌注组腹腔注射脂联素50μg/kg。比较各组血清ALT、AST、TBIL、ALP水平及肝脏组织中HE染色、免疫组化标记表达。
结果：缺血再灌注组、脂联素-缺血再灌注组ALT、AST、TBIL、ALP水平均较正常组显著升高（P<0.05），而缺血再灌注-脂联素组ALT、AST、TBIL、ALP水平显著低于缺血再灌注组和脂联素-缺血再灌注组（P<0.05）。HE染色显示，缺血再灌注组、脂联素-缺血再灌注组有较明显的肝细胞坏死、空泡变性和炎性细胞浸润，而缺血再灌注-脂联素组较少见到这种病理变化。免疫组化结果表明，缺血再灌注组、脂联素-缺血再灌注组NF-κB、TNF-α、IL-6标记表达较强，而缺血再灌注-脂联素组较强表达HSP70。
结论：脂联素预处理可一定程度上减轻大鼠肝脏缺血再灌注胆管损伤，可能与脂联素对HSP70的促进作用有关。
关键词：脂联素、肝脏、缺血再灌注、胆管损伤、HSP70
Abstract
Objective:Toinvestigatetheprotectiveeffectofadiponectinpretreatmentonbileductinjuryinratliverischemia-reperfusion.
Methods:FiftymaleSDratswererandomlydividedintofivegroups(n=10):normalgroup,ischemia-reperfusiongroup,adiponectingroup,adiponectin-ischemia-reperfusiongroup,ischemia-reperfusion-adiponectingroup.Theischemia-reperfusiongroup,adiponectin-ischemia-reperfusiongroup,andischemia-reperfusion-adiponectingroupunderwenthepaticportalocclusionfor20minutesandreperfusionfor60minutes;adiponectingroupandadiponectin-ischemia-reperfusiongroupwereintraperitoneallyinjectedwithadiponectin50μg/kg.ThelevelsofserumALT,AST,TBIL,ALPandtheexpressionofHEstainandimmunohistochemicalmarkersinlivertissueswerecomparedamongthegroups.
Results:ThelevelsofALT,AST,TBIL,andALPintheischemia-reperfusiongroupandadiponectin-ischemia-reperfusiongroupweresignificantlyincreasedcomparedwiththenormalgroup(P<0.05),whilethoseintheischemia-reperfusion-adiponectingroupweresignificantlylowerthanthoseintheischemia-reperfusiongroupandadiponectin-ischemia-reperfusiongroup(P<0.05).HEstainingshowedthattheischemia-reperfusiongroupandadiponectin-ischemia-reperfusiongrouphadmoreobviouslivercellnecrosis,vacuolardegenerationandinflammatorycellinfiltrate,whiletheischemia-reperfusion-adiponectingrouphadlesssuchpathologicalchanges.ImmunohistochemicalresultsshowedthatNF-κB,TNF-αandIL-6labelingexpressionwerestrongerintheischemia-reperfusio