甘草酸二铵对脂多糖所致小鼠急性肺损伤保护作用的研究.docx

甘草酸二铵对脂多糖所致小鼠急性肺损伤保护作用的研究
摘要
目的：探究甘草酸二铵（GA）对脂多糖（LPS）所致小鼠急性肺损伤（ALI）的保护作用及其机制。
方法：将40只SD大鼠随机分为对照组（C组）、模型组（M组）、低剂量GA组（L组）和高剂量GA组（H组），除C组外其余组细胞外基质（ECM）通过气管注入LPS建立ALI模型。L、H组分别给予10mg/kg、30mg/kgGA处理。24小时后记录大鼠体重、血气、肺湿重/干重比和超表观结构。
结果：ALI大鼠模型建立后，M组体重下降、肺湿重/干重比和动脉血气的肺泡动脉氧分压差（PAO2/FiO2）均明显高于C组（P<0.05）。低、高剂量GA均能显著减轻ALI大鼠的上述指标异常，且高剂量GA组显著优于低剂量GA组（P<0.05），提示GA对ALI大鼠的损伤具有保护作用。肺组织超表观结构显示L、H组肺泡充气减少，肺泡间隔变宽和破坏明显改善。L组肺泡浸润炎细胞多见，而H组抑制了炎症细胞浸润迅速，肺泡变态反应显著减轻。GA处理可减轻LPS对肺泡壁的破坏、减轻炎症反应、减少白细胞浸润。
结论：甘草酸二铵对LPS所致ALI大鼠模型有良好的保护作用，其机制可能与减轻肺泡壁破坏、减少炎症反应和白细胞浸润有关。
关键词：甘草酸；脂多糖；急性肺损伤；大鼠；保护作用
Abstract
Objective:ToexploretheprotectiveeffectandmechanismofGlycyrrhizinicAcid(GA)onlipopolysaccharide(LPS)-inducedacutelunginjury(ALI)inmice.
Methods:40SDratswererandomlydividedintothecontrolgroup(Cgroup),themodelgroup(Mgroup),thelow-doseGAgroup(Lgroup)andthehigh-doseGAgroup(Hgroup).ECMwasinjectedintothetracheatoestablishanALImodel.TheLandHgroupsweretreatedwith10mg/kgand30mg/kgGA,respectively.After24hours,theweight,bloodgas,lungwet-to-dryweightratioandultrastructuralchangesofthelungswererecorded.
Results:AftertheALImodelwasestablished,theMgroupshowedasignificantdecreaseinbodyweight,anincreaseinthelungwet-to-dryweightratio,andanincreaseinthepulmonaryarteryoxygenpressuredifference(PAO2/FiO2)comparedwiththeCgroup(P<0.05).Bothlow-andhigh-doseGAcouldsignificantlyreducetheabovementionedabnormalindicatorsofALIrats.Moreover,thehigh-doseGAgroupwassignificantlybetterthanthelow-doseGAgroup(P<0.05),suggestingthatGAhasaprotectiveeffectontheinjuryofALIrats.TheultrastructuralchangesoflungtissueinLandHgroupsshowedthatthealveolarinflationwasreduced,thealveolarseptumwaswidenedanddamaged,andthepathologicalchangeswereimproved.Lgroupshowedahighlevelofinflammatorycellinfiltration,whileHgroupinhibitedtherapidinfiltrationofinflammatorycells,andsignificantlyreducedpulmonaryallergicreactions.GAtreatmentcanalleviatelungtissuedamage,reducetheinflammatoryreaction,andreduceleukocyteinfiltrationcausedbyLPS.
Conclusion:GAhasagoodprotectiveeffectonthe