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玉米自交系H21遗传背景的染色体片段代换系构建及产量相关性状QTL定位.docx

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玉米自交系H21遗传背景的染色体片段代换系构建及产量相关性状QTL定位 Title:ConstructionofChromosomeSegmentSubstitutionLineswithGeneticBackgroundofMaizeInbredLineH21andQTLMappingofYield-RelatedTraits Abstract: Theidentificationandutilizationofquantitativetraitloci(QTL)isessentialforbreedinghigh-yieldingmaizevarieties.Inthisstudy,weconstructedchromosomesegmentsubstitutionlines(CSSLs)harboringthegeneticbackgroundofmaizeinbredlineH21byintrogressingchromosomalsegmentsfromahigh-yieldingelitemaizelineintotheH21genome.TheobjectiveofthisstudywastomapQTLsassociatedwithyield-relatedtraitsusingtheCSSLpopulation.Theresultsprovidevaluableinformationformarker-assistedselectionandbreeders'effortstoimprovemaizeyield. Introduction: Maize(ZeamaysL.)isoneofthemostimportantstaplecropsglobally.Improvingmaizeyieldiscrucialforensuringfoodsecurityandmeetingtheincreasingdemandformaizeproducts.QTLmappingisapowerfultoolforidentifyinggenomicregionsassociatedwithyield-relatedtraitsandprovidesusefulinsightsforgeneticimprovementofcrops.Inthisstudy,weaimedtoconstructCSSLswiththegeneticbackgroundofmaizeinbredlineH21andmapQTLsrelatedtoyield. Methods: 1.ConstructionofCSSLs: TheCSSLpopulationwascreatedbybackcrossinganelitehigh-yieldingmaizelinewiththemaizeinbredlineH21.TheresultingBC1F1plantswereself-pollinatedtodevelopBC1F2populations.SelectedBC1F2individualswererepeatedlybackcrossedwithH21toproduceBC3F1individuals.Finally,100BC3F1lineswithminimaldonorchromosomalsegmentswereselectedtodeveloptheCSSLpopulation. 2.PhenotypicEvaluation: TheCSSLpopulationandparents(H21andtheelitemaizeline)wereevaluatedforkeyyield-relatedtraits,includingplantheight,daystosilkemergence,earlength,kernelrowsperear,kernelsperrow,andgrainyieldperplantundermultipleenvironments.ThedatawereanalyzedusingappropriatestatisticalmethodstodeterminethesignificantdifferencesinthetraitsamongtheCSSLsandparents. 3.MolecularMarkerAnalysis: Molecularmarkers,suchassimplesequencerepeats(SSRs)andsinglenucleotidepolymorphisms(SNPs),wereusedtogenotypetheCSSLpopulationandparents.The