无忧汤对抑郁模型大鼠海马BDNF和TrkB表达的实验研究.docx

无忧汤对抑郁模型大鼠海马BDNF和TrkB表达的实验研究
摘要：
抑郁症是一种常见的精神障碍，现有的药物治疗存在一定的局限性，因此寻找有效的药物是非常必要的。无忧汤是一种中药方剂，常用于治疗抑郁症，但其作用机制尚不完全明确。本实验研究了无忧汤在抑郁模型大鼠海马BDNF和TrkB表达上的影响，结果表明，无忧汤能够提高大鼠海马BDNF和TrkB的表达，具有一定的抗抑郁作用。
关键词：无忧汤；抑郁症；BDNF；TrkB；海马
Introduction
抑郁症是一种常见的精神障碍，其主要表现为情绪低落、失眠、食欲减退等。目前抗抑郁药物的应用十分普遍，但其疗效不尽如人意，并且存在一定的副作用。因此，寻找有效的治疗抑郁症的药物是非常有必要的。
无忧汤是一种中药方剂，常用于治疗抑郁症，但其具体作用机制尚不明确。Studieshaveshownthatbrain-derivedneurotrophicfactor(BDNF)andtyrosinekinasereceptorB(TrkB)arecloselyrelatedtothepathogenesisofdepression.BDNFandTrkBareimportantmolecularmarkersofsynaptogenesisandneuroplasticity,andareinvolvedinregulatingthegrowthanddevelopmentofneurons.Therefore,thisstudyaimedtoinvestigatetheeffectofWuyoudecoctionontheexpressionofBDNFandTrkBinthehippocampusofdepressedmodelrats,andtoexploreitsmechanismofaction.
MaterialsandMethods
Experimentalanimals
60maleSDratswererandomlydividedinto6groups:normalgroup,modelgroup,fluoxetinegroup,andlow,medium,andhighdosegroupsofWuyoudecoction.Theratsinthemodelgroupweresubjectedtochronicunpredictablemildstresstoestablishadepressionmodel.
Drugadministration
Theratsinthelow,medium,andhighdosegroupsofWuyoudecoctionwereintragastricallyadministeredwith2.5g/kg,5g/kgand10g/kgofWuyoudecoctionrespectivelyfor4weeks.Fluoxetinegroupratswereorallyadministeredwith10mg/kgfluoxetine(Sigma,USA)for4weeks.Thenormalgroupandmodelgroupweregivenequaldosesofnormalsaline.
Behavioraltests
Openfieldandsucrosepreferencetestswereperformedtoevaluatethebehavioralchangesofratsbeforeandafterdrugadministration.Theopen-fieldtestevaluatestheexploratorybehaviorofratswhilethesucrosepreferencetestevaluatestheirinterestinsugarsolution.
Westernblot
Aftersacrificingtherats,theirhippocampustissuewascollectedforwesternblotanalysis,andtheexpressionofBDNFandTrkBweredetectedusingspecificantibodies.
Results
BehavioraltestresultsshowedthatthefluoxetineandWuyoudecoctiongroupshadsignificantlyhigherscoresintheopenfieldtestandsucrosepreferencetestcomparedtothemodelgroup.WesternblotanalysisshowedthattheexpressionofBDNFandTr