利用随机整合和基因置换策略在瑞氏木霉中异源表达栓菌漆酶的研究.docx

利用随机整合和基因置换策略在瑞氏木霉中异源表达栓菌漆酶的研究
Title:RandomIntegrationandGeneShufflingStrategiesinHeterologousExpressionofLaccaseEnzymeinTrichodermareesei
Abstract:
Laccaseenzymesarewidelyusedinvariousindustriesduetotheirversatilecatalyticpropertiesandpotentialapplications.HeterologousexpressionoflaccaseenzymesinTrichodermareeseihasgainedsignificantattentionasitoffersapromisingplatformforenhancedproductionandeasyscale-up.Inthisstudy,weinvestigatedtheapplicationofrandomintegrationandgeneshufflingstrategiestoenhanceheterologousexpressionoflaccaseenzymesinT.reesei.Ourfindingsindicatethatthesestrategieshavethepotentialtoimprovelaccaseproductionandfacilitateindustrialapplications.
Introduction:
Laccaseenzymesarecopper-containingoxidasesthatplayacrucialroleinlignindegradationandotherimportantprocessesinnature.Duetotheirremarkablecatalyticproperties,laccasesareextensivelyusedinvariousindustries,includingbiofuelproduction,textiledyeremoval,andpharmaceuticalsynthesis.However,thelimitedavailabilityandhighcostofnativelaccasesourceshaveledtoincreasedinterestinheterologousexpressionsystems.Trichodermareesei,anefficientcellulaseproducer,hasemergedasapromisinghostforheterologousexpressionoflaccases.
RandomIntegrationStrategy:
RandomintegrationinvolvestheintroductionofforeignDNAintothegenomeofthehostorganism,resultinginitsintegrationintorandomlocations.Thisstrategyallowsfortheexpressionofthedesiredgenefromitsendogenousregulatoryelements,minimizingtheneedforadditionaloptimizationsteps.InthecontextoflaccaseexpressioninT.reesei,randomintegrationhasshownpromisingresultsinenhancingenzymeproduction.Thismethodallowsfortheinsertionofmultiplecopiesofthelaccasegeneintothegenome,therebyincreasingtheoverallproductionlevels.Furthermore,randomintegrationpromotesgeneticdiversity,whichcanleadtothedevelopmentofstrainswithimprovedstabilityandperformance.
GeneShufflingStrategy:
Geneshufflingisapowerfultoolfordirectedevolutionandfunctionalimprovementofenzymes.Itinvolvesthecreationofadiverselibraryofmutatedgenesthroug