预览加载中，请您耐心等待几秒...

具有微纳交替结构的多层支架对骨髓间充质干细胞成骨分化的影响.docx

预览
1/2
2/2

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

具有微纳交替结构的多层支架对骨髓间充质干细胞成骨分化的影响 Title:EffectsofMulti-layeredScaffoldwithMicro-nanoAlternatingStructureonBoneDifferentiationofMesenchymalStemCellsinBoneMarrow Abstract: Bonetissueengineeringholdsgreatpromisefortheregenerationandrepairofdamagedordiseasedbonetissue.Scaffold-basedtherapyisacrucialcomponentofbonetissueengineering,asitprovidesthenecessarystructuralsupportandcuesforcellularattachment,proliferation,anddifferentiation.Theaimofthisstudyistoinvestigatetheeffectsofamulti-layeredscaffoldwithamicro-nanoalternatingstructureonthebonedifferentiationofmesenchymalstemcells(MSCs)inthebonemarrow. Introduction: Mesenchymalstemcells(MSCs)areanattractivecellsourceforbonetissueengineeringduetotheirself-renewalandmulti-lineagedifferentiationpotential.However,efficientosteogenicdifferentiationofMSCsiscriticalforsuccessfulboneregeneration.ScaffoldsystemswithappropriatestructuralandbiochemicalcueshavebeendevelopedtoguideMSCdifferentiationtowardstheosteogeniclineage.Themicro-nanoalternatingstructureofscaffoldshasshownpotentialforenhancingtheosteogenicdifferentiationofcells. Methods: Inthisstudy,amulti-layeredscaffoldwithamicro-nanoalternatingstructurewasfabricatedusingacombinationofmicrofabricationandnanofabricationtechniques.Thescaffoldwascomposedofalternatinglayersofamacro-porousscaffoldandananostructuredsurface.MSCswereseededontothescaffoldandculturedinosteogenicdifferentiationmedium. Results: Themulti-layeredscaffoldwithamicro-nanoalternatingstructurepromotedtheadhesion,proliferation,andosteogenicdifferentiationofMSCs.Themacro-porousscaffoldprovidedathree-dimensionalenvironmentforcelladhesionandproliferation,whilethenanostructuredsurfaceprovidednanotopographiccuesthatinfluencedcellbehavior.Themicro-nanoalternatingstructuresynergisticallyenhancedthebonedifferentiationofMSCscomparedtosingle-structuredscaffolds. Discussion: Theimprovedosteogenicdifferentiationobservedonthemulti-layeredscaffoldwithamicro-nanoalternatingstructurecanbeattributedtoseveralfactors.Firstly,themacro-poro