不同表型髓源抑制性细胞对哮喘小鼠气道炎症影响及免疫调节机制研究.docx

不同表型髓源抑制性细胞对哮喘小鼠气道炎症影响及免疫调节机制研究
摘要：
哮喘是一种泛指支气管气道慢性炎症性疾病，其发病机制尚未完全明确。目前认为哮喘的发病与遗传因素、环境因素、免疫调节失衡等多种因素有关。本研究旨在探究不同表型髓源抑制性细胞对哮喘小鼠气道炎症的影响及免疫调节机制。
实验将大鼠分为四组：正常对照组、哮喘模型组、骨髓抑制性细胞（MSCs）组和注射接种混合细胞的MSCs+Th2混合细胞组。通过比较不同组间气道炎症指标的变化情况，发现MSCs组和MSCs+Th2混合细胞组的气道炎症指标明显低于哮喘模型组，但低于正常对照组。血清中的IL-4、IL-5、IL-13等免疫调节因子的表达情况也进一步印证了此结果。此外，免疫组化染色显示，MSCs组和MSCs+Th2混合细胞组的气道中Treg细胞明显高于哮喘模型组，而CD4+T细胞的浸润情况和Foxp3表达水平均显著升高。
综上所述，本研究表明不同表型的MSCs对哮喘小鼠气道炎症有一定的抑制作用，并且可以通过调节免疫系统中Treg细胞的比例来实现。这一研究结果可能为哮喘的治疗提供新思路和方向。
关键词：哮喘；骨髓抑制性细胞；Treg细胞；免疫调节
Abstract:
Asthmaisachronicinflammatorydiseaseofthebronchialairways,thepathogenesisofwhichisnotfullyunderstood.Currently,itisbelievedthatthepathogenesisofasthmaisrelatedtogeneticfactors,environmentalfactors,andimmuneregulationimbalance.Thisstudyaimstoexploretheeffectofdifferentphenotypicmyeloidsuppressorcellsonairwayinflammationinasthmamiceandtheimmuneregulationmechanism.
Theratsweredividedintofourgroups:normalcontrolgroup,asthmamodelgroup,bonemarrowsuppressorcell(MSCs)group,andMSCs+Th2mixedcellgroup.Bycomparingthechangesinairwayinflammationindicatorsamongdifferentgroups,itwasfoundthattheairwayinflammationindicatorsintheMSCsgroupandMSCs+Th2mixedcellgroupweresignificantlylowerthanthoseintheasthmamodelgroup,butlowerthanthoseinthenormalcontrolgroup.TheexpressionofimmuneregulatoryfactorssuchasIL-4,IL-5,andIL-13intheserumfurtherconfirmedthisresult.Inaddition,immunohistochemistrystainingshowedthatTregcellsintheairwaysoftheMSCsgroupandMSCs+Th2mixedcellgroupweresignificantlyhigherthanthoseintheasthmamodelgroup,whiletheinfiltrationofCD4+TcellsandthelevelofFoxp3expressionweresignificantlyincreased.
Insummary,thisstudyshowsthatdifferentphenotypicMSCshaveacertaininhibitoryeffectonairwayinflammationinasthmamiceandcanregulatetheproportionofTregcellsintheimmunesystem.Theseresearchresultsmayprovidenewideasanddirectionsforthetreatmentofasthma.
Keywords:Asthma,myeloidsuppressorcells,Tregcells,immuneregulation