预览加载中，请您耐心等待几秒...

BVDVIBRV主要抗原表位基因串联表达及免疫原性研究.docx

预览
1/2
2/2

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

BVDVIBRV主要抗原表位基因串联表达及免疫原性研究 Introduction Bovineviraldiarrheavirus(BVDV)isahighlycontagiousRNAvirusthatbelongstothefamilyFlaviviridae.Thevirusiscommonincattlepopulationsworldwide,andcancausesignificanteconomiclossesduetodecreasedproductivityandincreasedmortality.BVDVisclassifiedintotwobiotypesbasedonitseffectsontheimmunesystemofcattle;cytopathic(CP)andnoncytopathic(NCP).TheCPvirusishighlyvirulent,whiletheNCPvirusislesspathogenicbutcancausepersistentinfectionsthatleadtoimmunosuppressionandincreasedsusceptibilitytosecondaryinfections.ThemajorantigenicepitopeofBVDVistheglycoproteinE2,whichplaysasignificantroleinthevirus'spathogenesisandthehost'simmuneresponse. BVDVIBRVAntigenicEpitope TheBVDVIBRVantigenicepitopeisimportantforunderstandingthevirus-hostinteractionanddevelopingeffectivestrategiesforcontrollingthedisease.TheglycoproteinE2ofBVDVIBRViscomposedofthreedomains:domainA,whichcontainstheantigenicepitope;domainB,whichisareceptorbindingdomain;anddomainC,whichisessentialforvirusfusionandentry.TheantigenicepitopeofBVDVIBRVisresponsibleforbindingtothehost'simmunesystemcellsandinitiatinganimmuneresponse.TheE2proteinishighlyvariableindifferentstrainsofBVDV,andtheantigenicepitopeislocatedatthetopofdomainA. TheBVDVIBRVantigenicepitopeisrecognizedbythehost'simmunesystemandelicitsanimmuneresponse.Theprimaryresponseistheproductionofneutralizingantibodiesthatbindtothevirusandpreventitfromenteringhostcells.Thehost'simmunesystemalsogeneratesT-cells,whichcanrecognizeanddestroyvirus-infectedcells. GeneConcatenation BVDVIBRVgeneconcatenationreferstothejoiningofmultiplegenesintoasingleDNAmoleculeforexpressioninahostcell.Geneconcatenationiscommonlyusedintheproductionofrecombinantproteinsandvaccines.Theadvantagesofgeneconcatenationincludehigherexpressionlevels,simplificationofthecloningprocess,andimprovedstabilityoftherecombinantprotein. BVDVIBRVgeneconcatenationcanbeusedtoexpresstheglycoproteinE2inahostcell.TheE2geneisamplifiedfromBVDVIBRVRNAandinsertedintoaplasmidvector.MultipleE2gen