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应用响应面法优化发酵培养基提高达托霉素产量.docx

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应用响应面法优化发酵培养基提高达托霉素产量 Title:OptimizationofFermentationMediumusingResponseSurfaceMethodologytoEnhanceDaunorubicinProduction Abstract: Daunorubicin,asecondarymetaboliteproducedbyStreptomycespeucetius,hassignificantanticancerproperties.Thepresentstudyaimedtooptimizethefermentationmediumusingresponsesurfacemethodologytoimprovedaunorubicinproduction.Acentralcompositedesign(CCD)wasemployedtoevaluatetheeffectsofthreeindependentvariables,namelyglucoseconcentration,peptoneconcentration,andinitialpHondaunorubicinyield.TheresultsindicatedthatglucoseconcentrationandinitialpHhadsignificantpositiveeffectsondaunorubicinproduction,whilepeptoneconcentrationshowedaquadraticeffect.Theoptimalconditionsformaximumdaunorubicinproductionweredeterminedtobe25g/Lglucoseconcentration,10g/Lpeptoneconcentration,andaninitialpHof7.5. 1.Introduction: DaunorubicinisapotentantitumoragentderivedfromStreptomycespeucetius,whichhasdemonstratedhighefficacyintreatingseveralformsofcancer.However,thelownaturalyieldofdaunorubicinhampersitscommercialproduction.Theoptimizationoffermentationmediumthroughresponsesurfacemethodologyoffersapromisingapproachtoenhancedaunorubicinproduction.Thisstudyaimedtoidentifytheoptimalmediumcomposition,specificallyfocusingonglucoseconcentration,peptoneconcentration,andinitialpHtoimprovedaunorubicinyield. 2.MaterialsandMethods: 2.1MicroorganismandInoculumPreparation: StreptomycespeucetiuswasobtainedfromtheMicrobialCultureCollection(MCC)andmaintainedonagarslants.Theinoculumwaspreparedbytransferringaloopfulofsporemassintoaseedculturemediumandincubatingfor3daysat28°C. 2.2ExperimentalDesign: Acentralcompositedesign(CCD)withthreefactorsandfivelevelswasemployedtoevaluatetheeffectsofglucoseconcentration(X1),peptoneconcentration(X2),andinitialpH(X3)ondaunorubicinyield.Thedesignincluded20experimentalrunswitheightaxialpointsandsixcenterpoints. 2.3FermentationProcess: Thefermentationwascarriedoutin500mLErlenmeyerflaskscontaining100mLoftheoptimizedfermentationmedium.Theconditionsweresetat28°C