2型糖尿病大鼠早期肾损害的研究.docx

2型糖尿病大鼠早期肾损害的研究[摘要]目的:探讨在2型糖尿病(NIDDM)大鼠早期肾损害过程中血浆中降钙素基因相关肽(CGRP)、内皮素(ET)含量的变化规律为早期防治糖尿病肾病(DN)提供理论依据。方法:采用链脲菌素(STZ)造成大鼠糖耐量异常然后喂以高糖、高脂肪饲料10周造成2型糖尿病模型分别测成型前4、2周、成型时、成型后2、4周血浆中CGRP、内皮素(ET)含量的变化。结果:成型前4周血浆CGRP、ET无明显改变;而成型前2周、成型时、成型后2、4周血浆中CGRP进行性下降同时血浆中ET含量明显增加。结论:CGRP、ET相互作用共同参与DM肾损害的发生、发展。动态观察血浆CGRP、ET的变化对于早期发现DN及分析病情有重要意义。[关键词]降钙素基因相关肽;内皮素;糖尿病;糖尿病肾损害[中图分类号]R392.6[文献标识码]A[文章编号]1673-7210(2009)07(a)-025-02Studyofearlykidneydamageinratswithnon-insulindependentdiabetesmellitus(NIDDM)LICuiling1LIJinling2ZHANGWen3(GuangzhouWomenandChildren'sMedicalCenterGuangzhou510120China)[Abstract]Objective:TostudythechangesoflevelsofCGRPandETinserumofearlykidneydamageinratswithnon-insulindependentdiabetesmellitus(NIDDM).Weprovidedtheoreticbasisforpreventingearlydiabetickidneticdestroy.Methods:RatsweregivedSTZanddevelopedimpainedglucosetolerancethenwerefedwithhighfatandhighglucose.AftertenweeksNIDDMmodelswereformed.ThelevelsofCGRPandETwereassayedwhentheDMmodelsofratswereformedandbeforeandafteritatthe2ndandthe4thweeksrespectively.Results:TheconcentrationofCGRPandETdidnotobviouslyalterinserumatthe4thweekbeforethemodelswereformedwhiletheseindicatorsincreasinglydeclinedatthe2ndweekbeforewhenthemodelswereformedandatthe2ndand4thweeksaftermodelswereformed;meanwhiletheleveksofETinserumincreasedsignificantly.Conclusion:TheinteractionsofCGRPETinvolvedinthehappeninganddevelopmentofearlydiabetickidneydestroy.ThechangesofCGRPandETinserumhaveimportantsignificanceonearlydiscoveringandanalyzingDN.[Keywords]CGRP;ET;DM;Earlykidneydamage糖尿病(DM)是一组由遗传和环境等多种因素相互作用引起的临床综合征。根据目前资料显示全世界约有1亿例DM患者我国患者数已接近2000万。DN系DM全身微血管合并症之一。研究表明微血管功能的完整性依赖于血管收缩和舒张的平衡[1]其中CGRP是体内最强的舒血管多肽ET则为最强烈的血管内皮收缩因子它们共同参与DN的发生、发展过程其机制尚不完全清楚。目前大多数研究主要集中于DN中、晚期的动态变化及干预处理效应但在DM