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响应面法优化松茸多糖提取工艺研究.docx

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响应面法优化松茸多糖提取工艺研究 Title:OptimizationofExtractionProcessforPolysaccharidesfromTricholomamatsutakeusingResponseSurfaceMethodology Abstract: Tricholomamatsutake,commonlyknownasmatsutakemushroom,isahighlyvaluablemushroomspecies,renownedforitsrichnutritionalandmedicinalproperties,particularlyintermsofpolysaccharidecontent.Polysaccharidesextractedfrommatsutakemushroomshaveexhibitedvariousbioactiveproperties,includingantioxidant,immunomodulatory,antitumor,andantimicrobialactivities.Therefore,developinganefficientextractionprocesstoobtainhigh-qualitymatsutakepolysaccharidesisofgreatimportance.Inthisstudy,weemployedresponsesurfacemethodology(RSM)tooptimizetheextractionprocessforpolysaccharidesfromT.matsutake,aimingtomaximizethepolysaccharideyieldandenhanceitsbioactivity. Keywords:Tricholomamatsutake,polysaccharides,responsesurfacemethodology,extractionprocess,optimization 1.Introduction 1.1Background 1.2Objectives 2.MaterialsandMethods 2.1Materials 2.2Samplepreparation 2.3Extractionprocess 2.4Experimentaldesignanddataanalysis 3.ResultsandDiscussion 3.1Singlefactorexperiments 3.2Responsesurfacemethodology 3.3Optimizationofextractionprocess 4.Characterizationofextractedpolysaccharides 4.1Yielddetermination 4.2Physicochemicalproperties 4.3Antioxidantactivity 4.4Immunomodulatoryeffects 5.Conclusion 5.1Significanceofthestudy 5.2Futuredirections 1.Introduction 1.1Background Tricholomamatsutakeisahighlysought-aftermushroomspeciesduetoitsdeliciousflavorandpotentmedicinalproperties.AmongthevariousbioactivecompoundsfoundinT.matsutake,polysaccharideshavegainedattentionfortheirpotentialhealthbenefits.Polysaccharidesarelong-chaincarbohydratescomposedofmonosaccharideunits,andtheypossessawiderangeofbioactivities,includingantioxidant,immunomodulatory,andanticancereffects.Therefore,theextractionandoptimizationofpolysaccharidesfromT.matsutakecouldprovidevaluableinsightsintoitsapplicationsinfunctionalfoods,pharmaceuticals,andnutraceuticalindustries. 1.2Objectives Themainobjectiveofthisstudywast