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响应面法优化提取槟榔花总酚的研究.docx

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响应面法优化提取槟榔花总酚的研究 Title:OptimizationofTotalPhenolicCompoundsExtractionfromBetelFlower(PiperbetleL.)UsingResponseSurfaceMethodology Abstract: Theextractionofbioactivecompoundsfromnaturalsourceshasgainedconsiderableattentionduetotheirpotentialhealthbenefits.Betelflower(PiperbetleL.)isapopularplantknownforitstherapeuticproperties,includinghighcontentsoftotalphenoliccompounds.Inthisstudy,responsesurfacemethodology(RSM)wasemployedtooptimizetheextractionconditionsformaximumextractionoftotalphenoliccompoundsfrombetelflower.Acentralcompositedesign(CCD)wasemployedtoinvestigatetheeffectsofthreeindependentvariables,namelyextractiontime,extractiontemperature,andsolvent-to-materialratio.Thetotalphenoliccontent(TPC)wasconsideredastheresponsevariable.Theoptimizedconditionsweredetermined,andtheresultswerevalidatedthroughexperiments.ThestudydemonstratedtheeffectivenessofRSMinoptimizingtheextractionprocessoftotalphenoliccompoundsfrombetelflowertoobtainthemaximumyield. 1.Introduction: Betelflower,alsoknownasPiperbetleL.,isextensivelyusedintraditionalmedicineduetoitsvariouspharmaceuticalproperties.Thebetelflowerisknowntoberichinbioactivecompounds,particularlytotalphenoliccompounds,whichhavebeenfoundtopossessantioxidant,anti-inflammatory,andantimicrobialproperties.Extractionofthesephenoliccompoundsfrombetelfloweriscrucialtoharnesstheirpotentialtherapeuticbenefits.OptimizationofextractionconditionsusingRSMisessentialtomaximizetheyieldofphenoliccompoundsfrombetelflower,ensuringthedevelopmentofeffectiveextractiontechniques. 2.MaterialsandMethods: 2.1PlantMaterial: Freshbetelflowerwascollectedfromalocalfarmandcleanedthoroughlybeforeextraction. 2.2ExtractionProcedure: Thebetelflowerwassubjectedtoextractionusingdifferentexperimentalconditions.Threeindependentvariables,namelyextractiontime(X1),extractiontemperature(X2),andsolvent-to-materialratio(X3),wereinvestigatedaspertheCCDdesign.Theresponsevariable,totalphenoliccontent(TPC),wasdeterminedusingtheFolin-Ciocalteumethod.Thecentralcompositedesi