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GARP家族转录因子HINGE1参与水稻氮磷稳态调控的分子机制研究 Title:MolecularMechanismsofGARPFamilyTranscriptionFactorHINGE1inRiceNitrogenandPhosphorusHomeostasisRegulation Abstract: Nitrogenandphosphorusareessentialnutrientsforplantgrowthanddevelopment,andtheiravailabilitysignificantlyimpactsagriculturalproductivity.Efficientuptake,allocation,andutilizationofnitrogenandphosphorusrequirecoordinatedandtightlyregulatedmolecularmechanismsinplants.Inthisstudy,weinvestigatetheroleoftheGARPfamilytranscriptionfactorHINGE1inthemolecularregulationofnitrogenandphosphorushomeostasisinrice.Ourfindingsshedlightontheintricatepathwaysinvolvedinnutrientsensing,signaling,andgeneexpressionregulationmediatedbyHINGE1inresponsetonitrogenandphosphorusavailability.ThisresearchprovidesvaluableinsightsintothepotentialapplicationofHINGE1incropimprovementthroughoptimizednutrientutilization. Introduction(200words): Nitrogen(N)andphosphorus(P)arecrucialmacronutrientsrequiredforplantgrowthanddevelopment.Rice,oneofthemostimportantstaplecrops,ishighlydependentonnitrogenandphosphorussupplyforitsyieldandnutritionalquality.However,excessiveorinsufficientavailabilityofNandPcanleadtoimbalancesandenvironmentalpollution.Toovercomethesechallenges,understandingthemolecularmechanismsinvolvedinNandPhomeostasisregulationiscritical. TheGARPfamilytranscriptionfactorshavebeenimplicatedinvariousbiologicalprocessesinplants,includingnutrienthomeostasis.HINGE1,amemberoftheGARPfamily,hasbeenfoundtoplayaroleinriceresponsetonutrientavailability.PreviousresearchhasidentifiedHINGE1asakeyplayerintheregulationofNandPuptake,assimilation,andallocationprocessesinrice.However,thedetailedmolecularmechanismsunderlyingHINGE1-mediatednutrientsensingandsignalingremainlargelyunknown. MethodsandResults(600words): Inthisstudy,wefirstexaminedtheexpressionpatternofHINGE1inresponsetonitrogenandphosphorusavailability.OurresultsshowedthatHINGE1expressionwassignificantlyinducedunderconditionsofnitrogendeficiency,indicatingitspotentialroleinnitrogenhomeostasis.Similarl