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小鼠早期胚胎发育体轴形成和原肠运动 Axisformation&GastrulationEarlymousedevelopmentPostimplantationCelllineagesinearlymouseembryoMouseembryodevelopmentfromfertilizationtogastrulationTwopossiblemechanismsforthegenerationofmultiplecelltypesandpolarityDevCell16,398-410,2009Cellposition-dependentdifferentiationmediatedbyHipposignalingMouseembryodevelopmentfromfertilizationtogastrulationEarlymousedevelopment‘‘SaltandPepper’’expressionofNanogandGata6intheICMofE3.5blastocystsSegregationandrelocationofepiblastandPrEcellsMouseembryodevelopmentfromfertilizationtogastrulationEmergingasymmetryandestablishmentofanterior-posteriorpolarityintheE5.75mouseembryoDistalVEtoAVEOtx2VEcis-lacZAsymmetricexpressionofLefty1transgenesinperi-implantationmouseembryosTwotypesofLefty1+cellinmouseembryosbetweenE3.5andE4.5DistinctoriginsofDVEandAVEOriginsofthedorsalandanteriorvisceralendodermSignalingpathwaysinvolvedinA-PaxisdeterminationMouseembryodevelopmentfromfertilizationtogastrulationNervoussystemRapidproliferationduringgastrulation:dramaticsignalingandgeneexpressionchangesVE:visceralendoderm AVE:anteriorVE DVE:distalVEEMTatmouseprimitivestreakEMTatmouseprimitivestreakMousegastrulationandgermlayerformationMyocardinCelllineagesinearlymouseembryoEmbryoculturesystemfortime-lapsemovieacquisitionofnodemorphogenesisTime-lapseimagesofnodemorphogenesisThedisplacementmodelofendodermmorphogenesisinthemousegastrulaThedispersalmodelofendodermmorphogenesisinthemousegastrulaVisceralendoderm-derivedcellsareincorporatedintotheguttubeCelllineagesinearlymouseembryoDefinitiveendodermBraGeneexpressionandregionalizedallocationoftissueprogenitorsThemarkersofgermlayersFate-mappingofthedistalcapepiblastbycarbocyaninedyelabelingThedistalcapepiblastcellsmigratetotheanteriorneuroectodermDevelopment139,423-436(2012)Development139,423-436(2012)内胚层InvivoICM/epiblast (E3.5-4.5)InvitroPS E6.0TheanteriorpartofepiblastsatE6.5andE7.5doesnothaveectodermdifferentiationpotentialTheanteriorepiblastinE7.0mouseembryohasthepotentialtogiverisetoneural