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基于DNA条形码及实时荧光定量PCR技术的燕窝鉴别研究.docx

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基于DNA条形码及实时荧光定量PCR技术的燕窝鉴别研究 Abstract Theidentificationofswiftletnestsiscrucialforensuringthesafetyandefficacyoftheiruseintraditionalmedicineandculinarypractices.Inthisstudy,weemployedDNAbarcodingandreal-timefluorescencequantitativePCRtechniquestoidentifythespeciesofswiftletnests.ByanalyzingthemitochondrialCOIgeneandITS2region,wewereabletodistinguishbetweenthethreemostcommonlyusedswiftletspecies,namelyAerodramusfuciphagus,Aerodramusmaximus,andCollocaliafuciphaga.Moreover,wedevelopedareal-timefluorescencequantitativePCRassaybasedonTaqManprobetechnologytoquantifyanddetecttheswiftletspeciesDNAinsamples.Ourstudyprovidesausefultoolforswiftletnestidentificationandqualitycontrol,whichcanreducetheriskofmislabelingandadulterationinthemarket. Introduction Swiftletnests,alsoknownasbird'snests,arehighlyvaluableintraditionalmedicineandculinarypractices,particularlyinSoutheastAsia.Theyaremadeofsalivaandothermaterialsproducedbyswiftlets,agroupofsmall,insect-eatingbirds.Thenestsarecomposedoffibersthatgluetogetherandformasolidstructure,whichisthenharvestedandsoldinavarietyofforms,suchasraw,dry,orprocessed. Thereareseveralspeciesofswiftletsthatproducenests,withthemostcommonlyusedspeciesbeingAerodramusfuciphagus,Aerodramusmaximus,andCollocaliafuciphaga.However,theidentificationofswiftletnestspeciesisdifficult,astheyaremorphologicallysimilarandtheneststhemselvescanbeprocessedinvariousways,makingitachallengetoidentifyauthenticityandpurity. TheuseofDNAbarcodingandreal-timefluorescencequantitativePCR(qPCR)techniquescanprovideapotentialsolutiontothisproblem.DNAbarcodingisamoleculartechniquethatusesashortDNAsequencefromastandardizedregionofthegenometoidentifyandclassifyspecies.Real-timefluorescenceqPCRisaquantitativemethodthatallowsforthedetectionandquantificationofspecificDNAsequencesinasample. Inthisstudy,weaimedtoidentifytheswiftletspeciesinprocessedandunprocessednestsamplesusingDNAbarcodinganddevelopareal-timefluorescenceqPCRassayforswiftletnestspeciesidentificationandqualitycontrol. Mat