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基于16SrRNA基因序列分析受砷和硫酸盐污染的土壤细菌多样性(英文).docx

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基于16SrRNA基因序列分析受砷和硫酸盐污染的土壤细菌多样性(英文) Title:AnalysisofSoilBacterialDiversityAffectedbyArsenicandSulfateContaminationBasedon16SrRNAGeneSequences Abstract: Soilpollutioncausedbyheavymetalsandsulfateposesasignificantthreattoenvironmentalandhumanhealth.Inthisstudy,weaimedtoinvestigatetheeffectsofarsenicandsulfatecontaminationonsoilbacterialdiversityusing16SrRNAgenesequencing.Soilsampleswerecollectedfromcontaminatedanduncontaminatedsites,andmicrobialDNAwasextracted.AmplificationandsequencingoftheV4regionofthe16SrRNAgenewereperformed,followedbybioinformaticsanalysistoexplorethebacterialdiversityandcommunitystructure. Introduction: Soilpollutionwithheavymetals,suchasarsenic,andhighlevelsofsulfateisamajorconcernworldwideduetotheirtoxiceffectsandpersistenceintheenvironment.Microorganismsplayacrucialroleinbiogeochemicalcyclingandtheremovalofcontaminantsfromsoil.Therefore,studyingsoilbacterialdiversityandcompositionundersuchstressorsisimportantforunderstandingtheimpactofpollutionanddevelopingeffectiveremediationstrategies. Methods: 1.Samplecollection:Soilsampleswerecollectedfrombothcontaminatedanduncontaminatedsites. 2.DNAextraction:MicrobialDNAwasextractedfromthesoilsamplesusingacommercialDNAextractionkit. 3.Polymerasechainreaction(PCR)amplification:TheV4regionofthe16SrRNAgenewasamplifiedusinguniversalprimers. 4.Sequenceanalysis:ThePCRampliconsweresequencedusingaNextGenerationSequencingplatform,andrawsequenceswerefilteredandtrimmedtoobtainhigh-qualitysequences. 5.Bioinformaticsanalysis:OperationalTaxonomicUnits(OTUs)wereclusteredandtaxonomicassignmentswereperformedusingbioinformaticstools. 6.Diversityanalysis:AlphadiversitymetricssuchasShannonindex,Simpsonindex,andChao1richnessestimatorwerecalculated. 7.Betadiversityanalysis:Principalcoordinatesanalysis(PCoA)andnon-metricmultidimensionalscaling(NMDS)wereperformedtoevaluatethedifferencesinbacterialcommunitystructurebetweencontaminatedanduncontaminatedsites. Results: Theanalysisofbacterialdiversityshowedasignificantdecreaseinspec