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响应面法优化天山岩黄芪多糖提取工艺.docx

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响应面法优化天山岩黄芪多糖提取工艺 Title:OptimizationoftheExtractionProcessofTianShanYanHuangQiPolysaccharidesUsingResponseSurfaceMethodology Abstract: TianShanYanisahighlyvaluedtraditionalChinesemedicinalherbknownforitstherapeuticproperties.ThepolysaccharidesderivedfromTianShanYanHuangQihavebeenofparticularinterestduetotheirpotentialhealthbenefits.Inthisstudy,weaimedtooptimizetheextractionprocessofTianShanYanHuangQipolysaccharidesusingtheresponsesurfacemethodology(RSM).TheRSMapproachinvolvesthedesign,analysis,andinterpretationofexperimentstofindtheoptimalsettingsandconditionsforagivenprocess.OurresultsdemonstratedtheeffectivenessofRSMindeterminingthekeyprocessvariablesandoptimizingtheextractionprocess,leadingtoimprovedpolysaccharideyieldandquality. 1.Introduction(150words) TianShanYan,scientificallyknownasAstragalusmembranaceus,isapopularmedicinalherbtraditionallyusedinChinesemedicine.Ithasbeenutilizedforitsnumeroushealthbenefits,includingimmunesystemenhancement,antioxidantactivity,andanti-inflammatoryeffects.Inrecentyears,particularattentionhasbeengiventothepolysaccharidesderivedfromTianShanYanHuangQiduetotheirpotentialpharmaceuticalandnutraceuticalapplications.However,theefficientextractionofthesepolysaccharidesremainsachallenge.Hence,theneedforoptimizationoftheextractionprocessarisestomaximizepolysaccharideyieldandmaintainitsquality.Thisstudyaimedtoexploretheresponsesurfacemethodology(RSM)foroptimizingtheextractionprocessofTianShanYanHuangQipolysaccharides. 2.MaterialsandMethods(250words) 2.1.ExperimentalPlan TheRSMapproachwasemployedtooptimizetheextractionprocess.TheBox-Behnkendesignwaschosenforexperimentaldesignduetoitsrobustnessandefficiency.Threeindependentvariables,namelyextractiontemperature(X1),extractiontime(X2),andliquid-to-materialratio(X3),wereselectedforinvestigation.Theresponsevariableswerepolysaccharideyield(Y1)andpolysaccharidecontent(Y2). 2.2.ExtractionProcedure TheextractionprocessinvolvedmacerationofTianShanYanHuangQipowderindistilledwater.Theextractiontemperat