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蚯蚓粪中高效解磷菌的筛选及解磷特性的研究.docx

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蚯蚓粪中高效解磷菌的筛选及解磷特性的研究 Title:ScreeningofHigh-EfficiencyPhosphate-SolubilizingBacteriainEarthwormVermicompostandStudyofPhosphate-SolubilizingCharacteristics Abstract: Phosphorus(P)isanessentialnutrientforplantgrowthanddevelopment.However,asignificantportionofphosphoruspresentinsoilsisininsolubleforms,makingitunavailabletoplants.Thisstudyaimstoinvestigatethescreeningprocessforhigh-efficiencyphosphate-solubilizingbacteria(PSB)inearthwormvermicompostandtoexaminetheirphosphate-solubilizingcharacteristics. Introduction: Phosphorusisavitalnutrientforplantgrowth,anditsavailabilityinsoilplaysacrucialroleinagriculturalproductivity.However,themajorityofphosphorusinsoilsispresentincomplex,insolubleforms,suchasphosphates.Therefore,theefficientutilizationofthisnutrientislimitedduetoitslowsolubility.Phosphate-solubilizingbacteria(PSB)aremicroorganismscapableofconvertinginsolublephosphateintosolubleformsthroughtheprocessofsolubilization.PSBplayasignificantroleinenhancingphosphorusavailabilitytoplantsbysecretingorganicacids,enzymes,andothermetabolites.Inthisstudy,wefocusedonthescreeningandcharacterizationofhigh-efficiencyPSBinearthwormvermicompost. Methods: 1.Collectionandpreparationofearthwormvermicompost:Freshvermicompostsampleswerecollectedandbroughttothelaboratory.Thesampleswerethoroughlymixed,stored,andkeptmoistatroomtemperatureuntilfurtherprocessing. 2.IsolationandscreeningofPSB:Thevermicompostsampleswereseriallydiluted,anddilutionswerespreadonselectivemediacontaininginsolublephosphateasthesolesourceofphosphorus.Plateswereincubatedatanappropriatetemperaturewithproperaeration.Afterincubation,coloniesshowingcharacteristicphosphate-solubilizinghalozoneswereselectedandpurifiedthroughsub-culturing. 3.CharacterizationofPSB:TheselectedPSBisolatesweresubjectedtovariousbiochemicaltests,includingphosphatesolubilizationefficiency,pHtolerance,andtolerancetootherabioticstressfactors.Additionally,moleculartechniquessuchas16SrRNAgenesequencingwereemployedfortaxonomicidentification. Results: Ba