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苦豆碱诱导自噬减轻球囊损伤后血管再狭窄.docx

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苦豆碱诱导自噬减轻球囊损伤后血管再狭窄 Title:TheRoleofColchicineinInducingAutophagytoMitigateVascularRestenosisafterBalloonInjury Abstract: Vascularrestenosis,apathologicalconditioncharacterizedbythere-narrowingofbloodvesselsafterballooninjury,significantlylimitsthelong-termsuccessofpercutaneoustransluminalangioplasty.Inrecentyears,autophagy,acellularprocessthatmaintainscellularhomeostasisbyremovingdamagedorganellesandproteins,hasemergedasapotentialtherapeutictargetforattenuatingrestenosis.Thisstudyaimstoinvestigatetheefficacyofcolchicine,aplant-derivedalkaloid,ininducingautophagyandmitigatingrestenosisafterballooninjury. Introduction: Ballooninjury-inducedneointimalhyperplasiaandsubsequentvascularrestenosisremainsamajorchallengeininterventionalcardiology.Theexcessiveproliferationandmigrationofsmoothmusclecells(SMCs)contributetotherestenoticprocess.WhilecurrenttreatmentoptionsfocusontargetingSMCgrowth,emergingevidencesuggeststhatmodulatingcellularautophagymaybeanovelapproachtopreventrestenosis.Autophagyhasbeenshowntoregulatecellsurvival,differentiation,andapoptosis,makingitanattractivetherapeutictarget.Inthisstudy,weinvestigatethepotentialofcolchicine,awell-knownanti-inflammatoryagent,toinduceautophagyandsuppressrestenosis. Methods: 1.AnimalModel:MaleSprague-Dawleyratswillbesubjectedtoballooninjury-inducedcarotidarteryrestenosismodel. 2.Groups:Ratswillbedividedintothreegroups:control,ballooninjurywithoutcolchicinetreatment,andballooninjurywithcolchicinetreatment. 3.ColchicineTreatment:Ratsinthecolchicinetreatmentgroupwillreceiveintraperitonealinjectionsofcolchicinedailyforthedurationofthestudy. 4.Assessment:Histologicalanalysiswillbeperformedtoevaluateneointimalhyperplasia,SMCproliferation,andextracellularmatrixdeposition.ImmunofluorescencestainingandWesternblottingwillassesstheexpressionofautophagy-relatedproteins.Quantitativereal-timePCRwillbeusedtomeasuretheexpressionofpro-inflammatorycytokines. 5.StatisticalAnalysis:Datawillbeanalyzedusingappropriatestatisticaltests,andp-values<0.