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红椿居群遗传多样性的SSR分析（英文）.docx

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红椿居群遗传多样性的SSR分析（英文） Title:SSRAnalysisRevealsGeneticDiversityinthePopulationofRedChinaberry(Meliaazedarach) Abstract: Thegeneticdiversityofaplantpopulationisafundamentalaspectofitsevolutionarypotentialandecologicaladaptability.Inthisstudy,weinvestigatedthegeneticdiversityoftheRedChinaberry(Meliaazedarach)populationthroughtheuseofSimpleSequenceRepeat(SSR)markers.SSRanalysisprovidedimportantinsightsintothegeneticstructure,geneflow,andevolutionaryhistoryofthespecies.OurresultshighlightedthehighgeneticdiversityandpotentialforadaptationwithintheRedChinaberrypopulation,emphasizingtheimportanceofconservationeffortstopreserveitsgeneticresources. Introduction: TheRedChinaberry(Meliaazedarach)isawidelydistributedtreespecieswitharichhistoryoftraditionalmedicinalandculturaluses.Thespeciesalsoexhibitsvariousecologicalroles,suchasprovidingshade,timber,andshelterforbirdsandinsectsalike.StudyingthegeneticdiversityofRedChinaberryiscrucialforunderstandingitsevolutionarypotential,populationdynamics,anddevisingeffectiveconservationstrategies.SSRmarkersarewidelyusedinpopulationgeneticsstudiesduetotheirco-dominantinheritance,highpolymorphismlevels,andeaseofdetection. Methods: Atotalof100RedChinaberryindividualsweresampledfromdifferentlocationsacrossitsdistributionrange.GenomicDNAwasextractedfromeachindividualandPCRamplificationwasperformedusingasetofselectedSSRmarkers.Theamplifiedproductswereseparatedonpolyacrylamidegelsandvisualizedusingstandardsilverstainingmethods.TheresultingSSRprofileswerescored,andgeneticdiversityparameterssuchasthenumberofalleles,allelicrichness,andgenediversitywerecalculated.Geneticstructureanalysiswasconductedtoassessthepopulationdifferentiation,geneflow,andgeneticclusteringusingsoftwareprograms. Results: OurSSRanalysisrevealedhighlevelsofgeneticdiversitywithintheRedChinaberrypopulation.AtotalofXallelesweredetected,withanaverageofXallelesperlocus.TheobservedandexpectedheterozygosityvalueswereXandX,respectively,indicatingahighlevelofgeneticvariability.Theallelicri