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用于高通量连续流细胞分离的介电泳芯片研究.docx

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用于高通量连续流细胞分离的介电泳芯片研究 Introduction Continuous-flowdielectrophoresis(cDEP)isatechniquewhichhasgainedsignificantattentioninrecentyearsduetoitspotentialforhigh-throughputcellseparation.Dielectrophoresis(DEP)isthemovementofchargedparticlesinanon-uniformelectricfield,anditcanbeutilizedtomanipulatecellsbasedontheirdielectricproperties.ThedevelopmentofcDEPmicrochipshasopenedupnewopportunitiesforefficientandrapidcellsorting,withapplicationsinareassuchascancerdiagnostics,rarecellisolation,anddrugdelivery. 1.PrincipleofContinuous-FlowDielectrophoresis TheprinciplebehindcDEPistoutilizethedifferenceinpolarizabilityandelectricalpropertiesbetweencellstomanipulatetheirmovementinamicrofluidicchannel.Whencellsaresubjectedtoanelectricfield,theelectricalforcesactingonthemresultineitherpositiveornegativeDEPdependingontheirphysicalproperties.PositiveDEPcausescellstomovetowardsregionsofhighelectricfieldstrength,whilenegativeDEPforcescellsawayfromtheseregions. 2.DesignandFabricationofcDEPMicrochips Toachievehigh-throughputcellseparation,cDEPmicrochipsaredesignedwithmultipleelectrodesandfluidchannels.Themicrochipsaretypicallymadeusingmaterialssuchasglassorpolymers,andtheelectrodesarepatternedonthesurfaceusinglithographytechniques.Themicrofluidicchannelsaredesignedtooptimizetheelectricfielddistributionandcellmanipulationefficiency.Theintegrationofelectrodesandfluidicchannelsallowsforcontinuoussampleflowandcellseparationinahigh-throughputmanner. 3.CellSeparationStrategiesusingcDEP VariousstrategieshavebeendevelopedtoachieveefficientcellseparationusingcDEPmicrochips.Onecommonapproachistoutilizethedifferenceindielectricpropertiesbetweentargetcellsandunwantedcells.Byapplyingappropriateelectricfieldconditions,targetcellscanbeselectivelymanipulatedwhileunwantedcellsareunaffected.Anotherstrategyinvolvestheuseofsize-basedseparation,wherecellsaresortedbasedontheirsize-dependentdielectrophoreticbehavior.Thisapproachisparticularlyusefulforisolatingrarecellsorcapturingspecificsubpopulationsofcells. 4.Ch