预览加载中，请您耐心等待几秒...

油茶树根际土壤解有机磷细菌的分离、鉴定及解磷能力分析（英文）.docx

预览
1/2
2/2

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

油茶树根际土壤解有机磷细菌的分离、鉴定及解磷能力分析（英文） Title:Isolation,Identification,andAnalysisofOrganicPhosphorus-DegradingBacteriaintheRhizosphereSoilofCamelliatenuifolia Abstract: Phosphorusisanessentialnutrientforplantgrowthanddevelopment,anditsavailabilityinthesoilisoftenlimited.Inorganicformsofphosphorus,suchasphosphate,areabundantinsoil,butplantshavelimitedabilitytoaccessthem.However,somebacteriahavetheabilitytosolubilizeandmineralizeorganicphosphorus,makingitavailableforplantuptake.Inthisstudy,weaimedtoisolate,identify,andanalyzeorganicphosphorus-degradingbacteriaintherhizospheresoilofCamelliatenuifolia,aplantknownforitshighphosphorusdemand. First,rhizospheresoilsampleswerecollectedfromCamelliatenuifoliaplantsgrowninoilteafields.ThesoilwasseriallydilutedandplatedonPikovskaya'sagarmediumsupplementedwithcalciumphytateasthesolesourceofphosphorus.Coloniesshowingclearzonesofhydrolysiswereselectedandfurtherpurified. Toidentifytheisolatedbacteria,morphological,physiological,andbiochemicaltestswereperformed.Gramstaining,motilitytests,andbiochemicalassaysincludingcatalaseandoxidasetestswerecarriedout.Additionally,the16SrRNAgenesequencingtechniquewasusedformolecularidentification.DNAextraction,PCRamplification,andsequencingwereconducted,andtheobtainedsequenceswerecomparedtothoseavailableinpublicdatabasesfortaxonomicclassification. Furthermore,theabilityoftheisolatedbacteriatodegradeorganicphosphoruswasassessedbyenzymeactivityassays.Extracellularphosphataseactivitywasdeterminedusingcolorimetricassays,wherep-nitrophenylphosphatewasusedasasubstrate.Thecultureswereincubated,andthereleasedp-nitrophenolwasquantifiedspectrophotometrically. PreliminaryresultsshowcasedthesuccessfulisolationofadiversecollectionofbacteriafromtherhizospheresoilofCamelliatenuifoliacapableofdegradingorganicphosphorus.Morphologicalandbiochemicalcharacterizationprovidedinsightsintothetaxonomyandmetaboliccharacteristicsofthesebacteria.Molecularidentificationusing16SrRNAgenesequencingfurtherrevealedtheirphylogeneticrelation