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沙丁鱼油中EPA和DHA富集工艺的研究.docx

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沙丁鱼油中EPA和DHA富集工艺的研究 Title:ResearchonEnrichmentTechniquesofEPAandDHAinSardineFishOil Introduction: Omega-3polyunsaturatedfattyacids(PUFAs)havegainedsignificantattentioninrecentyearsduetotheirpotentialhealthbenefits.Eicosapentaenoicacid(EPA)anddocosahexaenoicacid(DHA)aretwoessentialomega-3PUFAsfoundinfishoil,particularlysardinefishoil.However,thelowconcentrationandinstabilityofEPAandDHAinsardinefishoilnecessitatethedevelopmentofenrichmenttechniquestoincreasetheirlevels.ThispaperaimstodiscusstheresearchconductedonenrichmenttechniquesofEPAandDHAinsardinefishoil. Methods: 1.ExtractionTechniques: Severalextractionmethodscanbeusedtoobtainsardinefishoil,includingsolventextraction,supercriticalfluidextraction(SFE),andenzymaticextraction.ThechoiceofextractionmethodplaysacrucialroleinmaintainingthequalityandstabilityofEPAandDHA.Enzymaticextractionhasgainedpopularityasagentleandefficientmethodforpreservingthefattyacidprofileofsardinefishoil. 2.ConcentrationTechniques: Afterextraction,theconcentrationofEPAandDHAinsardinefishoilcanbeincreasedthroughvariousmethods. a)MolecularDistillation: Moleculardistillationinvolvesheatingthefishoilunderhighvacuumtoseparateandconcentratetheomega-3fattyacids.Thistechniqueisparticularlyeffectiveinremovingundesirablecompoundslikeheavymetals,resultinginahighlypureandconcentratedEPAandDHA. b)Fractionation: Fractionationtechniques,suchasureacomplexationandwinterization,canbeemployedtoisolateandconcentrateEPAandDHAfromsardinefishoil.Thesemethodsexploitthedifferentmeltingpointsandsolubilitiesofthefattyacidstoachieveseparation. c)SupercriticalFluidExtraction: Supercriticalfluidextraction(SFE)utilizescarbondioxideasthesolventtoextractandconcentrateEPAandDHA.SFEisapromisingtechniqueduetoitsabilitytoextractomega-3fattyacidswithoutdamagingtheirchemicalstructure,resultinginhighpurityandstability. 3.EnzymaticTransesterification: Enzymatictransesterificationisabioengineeringapproachthatenhancestheesterificationoffreefattyacidswithalcohol,resultinginahigherconc