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响应面优化双酶法提取马铃薯渣膳食纤维工艺.docx

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响应面优化双酶法提取马铃薯渣膳食纤维工艺 Title:OptimizationofPotatoResidueDietaryFiberExtractionUsingResponseSurfaceMethodology Abstract: Potatoresiduesareavaluablesourceofdietaryfiber.Theextractionofdietaryfiberfrompotatoresiduescanenhanceitsnutritionalvalueandcontributetothedevelopmentoffunctionalfoodproducts.Inthisstudy,responsesurfacemethodology(RSM)wasemployedtooptimizetheprocessparametersfortheextractionofdietaryfiberfrompotatoresiduesusingadual-enzymemethod.Theeffectsofenzymeconcentration,enzymeratio,extractiontemperature,andextractiontimeontheyieldofdietaryfiberwereinvestigated.Theresultsshowedthatthedual-enzymemethodsignificantlyincreasedtheyieldofdietaryfibercomparedtoconventionalmethods.RSManalysisrevealedthattheoptimalconditionsfordietaryfiberextractionwereanenzymeconcentrationof0.5%,anenzymeratioof1:1,anextractiontemperatureof50°C,andanextractiontimeof120minutes.Undertheseconditions,theyieldofdietaryfiberreached32.5%. 1.Introduction Potatoresidues,alsoknownaspotatopeelsorpotatopomace,arebyproductsofpotatoprocessing.Theyareabundantindietaryfiber,whichhasbeenshowntohavenumeroushealthbenefits,includingimprovedbowelfunction,decreasedriskofchronicdiseases,andweightmanagement.Therefore,theextractionofdietaryfiberfrompotatoresiduesisofgreatinterestinthedevelopmentoffunctionalfoodproducts. 2.MaterialsandMethods 2.1Materials Potatoresidueswerecollectedfromalocalpotatoprocessingplant.α-amylaseandcellulasewereobtainedfromcommercialsources. 2.2ExperimentalDesign Acentralcompositedesign(CCD)wasusedtoinvestigatetheeffectsofenzymeconcentration(X1),enzymeratio(X2),extractiontemperature(X3),andextractiontime(X4)ontheyieldofdietaryfiberfrompotatoresidues.Theindependentvariableswerecodedatthreedifferentlevels(-1,0,+1). 2.3ExtractionProcedure Potatoresidueswerefirstwashedanddried.Then,theyweregroundintoafinepowder.Thepowderwastreatedwithα-amylaseandcellulaseatthepredeterminedenzymeconcentrationsandratios.Theextractionwascarriedoutunderthespecifiedtemperatureandtimeconditions.Afterextraction,t