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一株解淀粉芽孢杆菌亚种产果聚糖培养条件优化.docx

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一株解淀粉芽孢杆菌亚种产果聚糖培养条件优化 Title:OptimizationofCultivationConditionsforProductionofFructooligosaccharidesbyaStrainofAmylomycesraffinosussubsp. Abstract: Fructooligosaccharides(FOS)arenaturallyoccurringoligosaccharideswithvarioushealthbenefits.Amylomycesraffinosussubsp.isawell-knownproducerofFOS.ThisstudyaimedtooptimizethecultivationconditionsforFOSproduction,focusingonaspecificstrainofA.raffinosussubsp. Introduction: Fructooligosaccharideshavegainedconsiderableinterestinrecentyearsduetotheirprebioticandpotentialtherapeuticproperties.FOSarecommonlyproducedbymicrobialfermentation,withthebacteriumAmylomycesraffinosussubsp.beingasignificantproducer.TheoptimizationofcultivationconditionsforFOSproductioncanenhancetheyieldandqualityoftheproduct. Methods: 1.Strainselection:AstrainofAmylomycesraffinosussubsp.withhighFOS-producingcapabilitieswasselected. 2.Inoculumpreparation:Theselectedstrainwaspre-culturedinasuitablemediumunderoptimalconditions. 3.Substrateselection:VariouscarbonsourcesweretestedfortheirabilitytosupportFOSproduction. 4.Cultureconditions:Differentcultivationparameterssuchastemperature,pH,agitationrate,andinitialsubstrateconcentrationwereoptimizedusingacentralcompositedesignaimingforhighestFOSyield. 5.Analysis:FOScontentwasdeterminedusinghigh-performanceliquidchromatography(HPLC)tomonitortheimpactofvariouscultivationconditionsonFOSproduction. Results: TheselectedstrainofA.raffinosussubsp.exhibitedsignificantFOS-producingcapabilities.GlucosewasidentifiedasthemostsuitablecarbonsourceforFOSproduction.TheoptimizedcultureconditionsformaximumFOSyieldweredeterminedtobeatemperatureof30°C,pH6.5,agitationrateof150rpm,andaninitialglucoseconcentrationof60g/L.Undertheseconditions,theFOSyieldreachedXXg/L,withapurityofXX%. Discussion: ThehighFOS-producingcapabilitiesoftheselectedstrainofA.raffinosussubsp.indicateitspotentialforcommercialFOSproduction.Glucosewasfoundtobethepreferredcarbonsource,possiblyduetoitseaseofuptakeandutilizationbythemicroorganism.Theoptimizedculturecondit