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不同品种苜蓿皂甙含量的测定分析研究.docx

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不同品种苜蓿皂甙含量的测定分析研究 Title:AnalysisandResearchontheDeterminationofSaponinContentinDifferentVarietiesofLucerne Abstract: Lucerne(MedicagosativaL.)isawidelycultivatedforagecropwell-knownforitshighnutritionalvalue.Amongitsvariousbioactivecompounds,saponinshaveattractedconsiderableattentionduetotheirdiversephysiologicaleffects.ThisstudyaimedtoanalyzeandresearchthevariationinsaponincontentamongdifferentvarietiesofLucerne. Introduction: Lucerneisaleguminousperennialplantcommonlyusedasaforagecropduetoitshighproteinandfibercontent.Itisalsoknownforitspotentialhealthbenefits,particularlyattributedtoitssaponincontent.Saponinsarenaturalplantglycosidesthatpossessawiderangeofbioactivities,suchasantimicrobial,anti-inflammatory,andanticancerproperties.ThedeterminationandanalysisofsaponincontentinLucernevarietiescanprovidevaluableinsightintotheirpotentialusesandapplications. Methods: 1.Samplecollection:AdiversesetofLucernevarietiesfromdifferentgeographicallocationswascollectedforthisstudy. 2.Preparationofextracts:ThewholeaerialpartsofLucerneplantswereair-driedandgroundintoafinepowder.Thepowderwasthensubjectedtosolventextractionusingasuitablesolvent(e.g.,methanolorethanol). 3.Quantificationofsaponincontent:ThesaponincontentintheLucerneextractswasdeterminedusingvariousanalyticaltechniques,suchasgravimetricmethod,spectrophotometry,orhigh-performanceliquidchromatography(HPLC).ThesemethodscanprovideaccuratemeasurementsofsaponincontentindifferentLucernevarieties. 4.Statisticalanalysis:TheobtaineddatawerestatisticallyanalyzedtoidentifyanysignificantdifferencesinsaponincontentamongtheLucernevarieties. Results: TheanalysisofsaponincontentindifferentLucernevarietiesrevealedsignificantvariation.Somevarietiesexhibitedhighersaponincontentthanothers,indicatinggeneticandenvironmentalinfluencesonsaponinbiosynthesis.ThequantificationofsaponinsusingHPLCshowedthepresenceofmultiplesaponincompoundswithvaryingconcentrationsamongtheLucernevarieties. Discussion: VariationinsaponincontentamongdifferentLucernev