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食蟹猴支持细胞分离、培养、鉴定及增殖与凋亡研究.docx

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食蟹猴支持细胞分离、培养、鉴定及增殖与凋亡研究 Title:CurrentResearchProgressonCellIsolation,Cultivation,Identification,Proliferation,andApoptosisofMacacafascicularis Abstract: Macacafascicularis,commonlyknownasthecrab-eatingmacaqueorlong-tailedmacaque,isawidelyusednon-humanprimatemodel.Overtheyears,thisspecieshasproventobeanexcellentmodelforstudyingvariousaspectsofhumandiseases,includingcellbiology,immunology,pharmacology,andtoxicology.Thispaperaimstoprovideacomprehensiveoverviewofthecurrentresearchprogressoncellisolation,cultivation,identification,proliferation,andapoptosisstudiesinMacacafascicularis,highlightingitsimportanceinbiomedicalresearch. Introduction: Non-humanprimatemodelsplayacrucialroleinacceleratingtranslationalresearch.Amongthenon-humanprimatespecies,thecrab-eatingmacaque(Macacafascicularis)isoneofthemostcommonlyusedmodelsduetoitsphysiologicalandgeneticsimilaritiestohumans.Cellisolationandculturetechniquesarefundamentalforstudyingthecellularmechanismsunderlyingvariousdiseasesanddrugresponses.Moreover,theidentification,proliferation,andapoptosisofcellsareimportantinunderstandingdiseaseprogressionandtherapeuticinterventions.Inthisreview,wewilldiscussthecurrentresearchprogressoncellisolation,cultivation,identification,proliferation,andapoptosisstudiesinMacacafascicularis. CellIsolation: TheisolationofcellsfromMacacafascicularisisacriticalstepforvariouscellularexperiments.Commonlyusedmethodsincludemechanicaldissociation,enzymaticdigestion,andgradientcentrifugation.Mechanicaldissociationinvolvesthephysicaldisruptionoftissuestoobtainsingle-cellsuspensions.Enzymaticdigestionutilizesenzymessuchascollagenase,trypsin,ordispasetobreakdownextracellularmatrixcomponentsandreleaseindividualcells.Gradientcentrifugationtechniques,suchasFicoll-Paquedensitygradientcentrifugation,areusedtoseparatedifferentcellpopulationsbasedontheirdensities. CellCultivation: Onceisolated,Macacafasciculariscellscanbecultivatedusingavarietyoftechniques.CommonlyusedcellculturemediaincludeDulbecco'sModifiedEagleM