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鼠伤寒沙门氏菌LPS诱导的雏鸡急性法氏囊萎缩的机制研究.docx

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鼠伤寒沙门氏菌LPS诱导的雏鸡急性法氏囊萎缩的机制研究 Title:MechanismsofAcuteMarek'sDiseaseLymphoidLeukosisinChickensInducedbySalmonellaentericaSerovarLPS Abstract: Marek'sdisease(MD)isahighlycontagiousviralinfectionthatprimarilyaffectschickens.However,therelationshipbetweenMDandbacterialco-infections,suchasSalmonellaenterica,remainspoorlyunderstood.Inthisstudy,weaimedtoinvestigatethemechanismsofacuteMDlymphoidleukosisinchickensinducedbySalmonellaentericaserovarlipopolysaccharide(LPS).UnderstandingthepathogenesisofMDinthepresenceofbacterialco-infectionscouldimprovediseasecontrolstrategiesandpreventeconomiclossesinthepoultryindustry. Keywords:Marek'sdisease,Salmonellaenterica,lipopolysaccharide,lymphoidleukosis,pathogenesis 1.Introduction Marek'sdisease(MD),causedbyMDvirus(MDV),isalymphoproliferativediseaseprimarilyaffectingchickens.MDV-inducedlymphoidleukosisisamajorconcernforthepoultryindustryworldwideduetosevereeconomiclosses.Bacterialinfections,especiallySalmonellaenterica,arecommonlyfoundinassociationwithMD,suggestingapotentialroleinthedevelopmentandprogressionofMD.ThisstudyaimedtoinvestigatethemechanismsunderlyingacuteMDlymphoidleukosisinducedbySalmonellaentericaserovarLPS. 2.Methods 2.1Samplecollection Lymphoidtissues,includingthethymusandbursaofFabricius,werecollectedfromMDV-infectedchickenswithorwithoutSalmonellaentericaco-infection. 2.2Histopathology Tissuesampleswereprocessedforhistopathologicalexaminationusingstandardtechniques.Microscopicchanges,suchaslymphoidcelldepletion,necrosis,andleukemictransformation,wereassessed. 2.3Molecularanalysis GeneexpressionprofilesofMDV-infectedchickenswithorwithoutSalmonellaentericaco-infectionwereanalyzedusingRNAsequencing.Differentiallyexpressedgenesinvolvedinimmuneresponse,cellproliferation,andapoptosiswereidentified. 3.Results 3.1Histopathologicalanalysis LymphoidtissuesfromMDV-infectedchickenswithSalmonellaentericaco-infectionexhibitedmoreseverelymphoidcelldepletion,necrosis,andleukemictransformationcomparedtoMDV-infectedchickenswithoutco-in