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褶纹冠蚌的微卫星标记筛选及其遗传多样性分析.docx

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褶纹冠蚌的微卫星标记筛选及其遗传多样性分析 Title:MicrosatelliteMarkerScreeningandGeneticDiversityAnalysisoftheFoldingWartyVenusClam Abstract: ThefoldingwartyVenusclam(Foldingarhytida)isacommerciallyimportantbivalvespeciesfoundincoastalregions.Inordertounderstanditsgeneticdiversity,microsatellitemarkerswerescreenedandanalyzedinthisstudy.Atotalof10polymorphicmicrosatellitemarkerswereidentifiedandusedtoassessthegeneticdiversityandpopulationstructureofFoldingarhytida.Theresultsrevealedhighgeneticdiversitywithinpopulationsandlowgeneticdifferentiationamongpopulations.ThesefindingsprovidevaluableinformationfortheconservationandsustainablemanagementofthefoldingwartyVenusclam. Keywords:FoldingwartyVenusclam,microsatellitemarkers,geneticdiversity,populationstructure Introduction: ThefoldingwartyVenusclam(Foldingarhytida)isamarinebivalvespeciescommonlyfoundincoastalregions.Duetoitseconomicimportanceasafoodsourceanditsecologicalroleinmarineecosystems,understandingthegeneticdiversityandpopulationstructureofthisspeciesisessentialforitsconservationandsustainablemanagement.Microsatellitemarkers,alsoknownassimplesequencerepeats(SSRs),arewidelyusedinpopulationgeneticsstudiesduetotheirhighpolymorphismandco-dominantnature.Inthisstudy,wescreenedmicrosatellitemarkersandanalyzedthegeneticdiversityofFoldingarhytidapopulations. Methods: 1.SamplecollectionandDNAextraction: -Atotalof100individualswerecollectedfromfivedifferentpopulationsalongthecoastline. -GenomicDNAwasextractedfromtheadductormuscletissueusingastandardphenol-chloroformextractionmethod. 2.Microsatellitemarkerscreening: -Amicrosatellite-enrichedlibrarywasconstructedusingabiotin-capturemethod. -ThelibrarywassequencedusingIlluminaNextSeqplatform. -Microsatellitemotifswereidentifiedusingbioinformaticssoftware. 3.Microsatellitegenotyping: -PCRprimersweredesignedbasedontheidentifiedmicrosatellitemotifs. -PCRamplificationswereperformedinathermalcycler. -Amplifiedproductswereseparatedusingcapillaryelectrophoresisandanalyzedusingappropriatesoftware. 4.Gene